ArticleBMC plant biology2011
Comparative analysis of expressed sequence tags (ESTs) between drought-tolerant and -susceptible genotypes of chickpea under terminal drought stress.
Article in BMC plant biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 107 citations in OpenAlex.
- Evaluation of Skin Color Supervision Genes in Chickpea Seeds by Multiomics.Molecular biotechnology · 2025Article
- Index-Based selection of Chickpea (Cicer arietinum L.) genotypes for enhanced drought tolerance.Scientific reports · 2025Article
- Alterations in starch, sucrose, and abscisic acid metabolism under drought stress in contrasting genotypes of chickpea.Journal of biosciences · 2025Article
- Genetic Databases and Gene Editing Tools for Enhancing Crop Resistance against Abiotic Stress.Biology · 2023Review
- Multi-Omics Pipeline and Omics-Integration Approach to Decipher Plant's Abiotic Stress Tolerance Responses.Genes · 2023Review
- Phyto-microbiome to mitigate abiotic stress in crop plants.Frontiers in microbiology · 2023Review
- Molecular Breeding and Drought Tolerance in Chickpea.Life (Basel, Switzerland) · 2022Review
- Combined Drought and Heat Stress Influences the Root Water Relation and Determine the Dry Root Rot Disease Development Under Field Conditions: A Study Using Contrasting Chickpea Genotypes.Frontiers in plant science · 2022Article
- Drought and Heat Stress in Cool-Season Food Legumes in Sub-Tropical Regions: Consequences, Adaptation, and Mitigation Strategies.Plants (Basel, Switzerland) · 2021Review
- Functional annotations of ESTs ofSaudi journal of biological sciences · 2021Article
- Gaining Insight into Exclusive and Common Transcriptomic Features Linked to Drought and Salinity Responses across Fruit Tree Crops.Plants (Basel, Switzerland) · 2020Article
- Biotechnological Perspectives of Omics and Genetic Engineering Methods in Alfalfa.Frontiers in plant science · 2020Review
- Transcriptome profiling illustrates expression signatures of dehydration tolerance in developing grasspea seedlings.Planta · 2019Article
- Transcript expression profiling in two contrasting cultivars and molecular cloning of a SKP-1 like gene, a component of SCF-ubiquitin proteasome system from mungbean Vigna radiate L.Scientific reports · 2019Article
- Research Progress and Perspective on Drought Stress in Legumes: A Review.International journal of molecular sciences · 2019Review
- Developing Climate-Resilient Chickpea Involving Physiological and Molecular Approaches With a Focus on Temperature and Drought Stresses.Frontiers in plant science · 2019Review
- Elucidating the role of osmotic, ionic and major salt responsive transcript components towards salinity tolerance in contrasting chickpea (Physiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Milestones achieved in response to drought stress through reverse genetic approaches.F1000Research · 2018Review
- Genotype-independentPlant methods · 2018Article
- Identification of low Ca(2+) stress-induced embryo apoptosis response genes in Arachis hypogaea by SSH-associated library lift (SSHaLL).Plant biotechnology journal · 2016Article
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChickpea (Cicer arietinum L.) is an important grain-legume crop that is mainly grown in rainfed areas, where terminal drought is a major constraint to its productivity. We generated expressed sequence tags (ESTs) by suppression subtraction hybridization (SSH) to identify differentially expressed genes in drought-tolerant and -susceptible genotypes in chickpea.
resultsEST libraries were generated by SSH from root and shoot tissues of IC4958 (drought tolerant) and ICC 1882 (drought resistant) exposed to terminal drought conditions by the dry down method. SSH libraries were also constructed by using 2 sets of bulks prepared from the RNA of root tissues from selected recombinant inbred lines (RILs) (10 each) for the extreme high and low root biomass phenotype. A total of 3062 unigenes (638 contigs and 2424 singletons), 51.4% of which were novel in chickpea, were derived by cluster assembly and sequence alignment of 5949 ESTs. Only 2185 (71%) unigenes showed significant BLASTX similarity (<1E-06) in the NCBI non-redundant (nr) database. Gene ontology functional classification terms (BLASTX results and GO term), were retrieved for 2006 (92.0%) sequences, and 656 sequences were further annotated with 812 Enzyme Commission (EC) codes and were mapped to 108 different KEGG pathways. In addition, expression status of 830 unigenes in response to terminal drought stress was evaluated using macro-array (dot blots). The expression of few selected genes was validated by northern blotting and quantitative real-time PCR assay.
conclusionOur study compares not only genes that are up- and down-regulated in a drought-tolerant genotype under terminal drought stress and a drought susceptible genotype but also between the bulks of the selected RILs exhibiting extreme phenotypes. More than 50% of the genes identified have been shown to be associated with drought stress in chickpea for the first time. This study not only serves as resource for marker discovery, but can provide a better insight into the selection of candidate genes (both up- and downregulated) associated with drought tolerance. These results can be used to identify suitable targets for manipulating the drought-tolerance trait in chickpea.
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