ArticleJournal of industrial microbiology & biotechnology2011
Metabolic engineering of Escherichia coli for improving L-3,4-dihydroxyphenylalanine (L-DOPA) synthesis from glucose.
Article in Journal of industrial microbiology & biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 76 citations in OpenAlex.
- Heterologous expression of hydroxylase coupled with pathway optimization enables highly efficient ʟ-DOPA production inSynthetic and systems biotechnology · 2026Article
- Efficient synthesis of l-DOPA inSynthetic and systems biotechnology · 2026Article
- Recent advances in metabolic engineering for microbial production of L-tyrosine.World journal of microbiology & biotechnology · 2026Review
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- Engineering non-P450 3-hydroxylase forSynthetic and systems biotechnology · 2025Article
- Directed evolution of L-DOPA sensing and production enables efficient incorporation of an expanded genetic code into proteins.bioRxiv : the preprint server for biology · 2025Article
- The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli.Microbial cell factories · 2025Article
- Expanding the application of tyrosine: engineering microbes for the production of tyrosine and its derivatives.Frontiers in bioengineering and biotechnology · 2025Review
- Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate-Oxaloacetate-Pyruvate-Derived Amino Acids.Molecules (Basel, Switzerland) · 2024Review
- Computer-aided design and implementation of efficient biosynthetic pathways to produce high added-value products derived from tyrosine inFrontiers in bioengineering and biotechnology · 2024Article
- Relationship Between Gut Bacteria and Levodopa Metabolism.Current neuropharmacology · 2023Review
- Advances in engineering microbial biosynthesis of aromatic compounds and related compounds.Bioresources and bioprocessing · 2021Review
- Cost-Effective Production of L-DOPA by Tyrosinase-Immobilized Polyhydroxyalkanoate Nanogranules in EngineeredMolecules (Basel, Switzerland) · 2021Article
- Oral berberine improves brain dopa/dopamine levels to ameliorate Parkinson's disease by regulating gut microbiota.Signal transduction and targeted therapy · 2021Article
- Chromosome Engineering To Generate Plasmid-Free Phenylalanine- and Tyrosine-OverproducingApplied and environmental microbiology · 2020Article
- A single amino acid substitution in aromatic hydroxylase (HpaB) of Escherichia coli alters substrate specificity of the structural isomers of hydroxyphenylacetate.BMC microbiology · 2020Article
- Recent Advances in Metabolically Engineered Microorganisms for the Production of Aromatic Chemicals Derived From Aromatic Amino Acids.Frontiers in bioengineering and biotechnology · 2020Review
- Integrating enzyme evolution and high-throughput screening for efficient biosynthesis of L-DOPA.Journal of industrial microbiology & biotechnology · 2019Article
- Metabolic engineering of Escherichia coli BL21 (DE3) for de novo production of L-DOPA from D-glucose.Microbial cell factories · 2019Article
- Metabolic engineering for improving L-tryptophan production in Escherichia coli.Journal of industrial microbiology & biotechnology · 2019Review
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6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
L-3,4-dihydroxyphenylalanine (L-DOPA) is an aromatic compound employed for the treatment of Parkinson's disease. Metabolic engineering was applied to generate Escherichia coli strains for the production of L-DOPA from glucose by modifying the phosphoenolpyruvate:sugar phosphotransferase system (PTS) and aromatic biosynthetic pathways. Carbon flow was directed to the biosynthesis of L-tyrosine (L-Tyr), an L-DOPA precursor, by transforming strains with compatible plasmids carrying genes encoding a feedback-inhibition resistant version of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, transketolase, the chorismate mutase domain from chorismate mutase-prephenate dehydratase from E. coli and cyclohexadienyl dehydrogenase from Zymomonas mobilis. The effects on L-Tyr production of PTS inactivation (PTS(-) gluc(+) phenotype), as well as inactivation of the regulatory protein TyrR, were evaluated. PTS inactivation caused a threefold increase in the specific rate of L-Tyr production (q( L-Tyr)), whereas inactivation of TyrR caused 1.7- and 1.9-fold increases in q( L-Tyr) in the PTS(+) and the PTS(-) gluc(+) strains, respectively. An 8.6-fold increase in L-Tyr yield from glucose was observed in the PTS(-) gluc(+) tyrR (-) strain. Expression of hpaBC genes encoding the enzyme 4-hydroxyphenylacetate 3-hydroxylase from E. coli W in the strains modified for L-Tyr production caused the synthesis of L-DOPA. One of such strains, having the PTS(-) gluc(+) tyrR (-) phenotype, displayed the best production parameters in minimal medium, with a specific rate of L-DOPA production of 13.6 mg/g/h, L-DOPA yield from glucose of 51.7 mg/g and a final L-DOPA titer of 320 mg/l. In a batch fermentor culture in rich medium this strain produced 1.51 g/l of L-DOPA in 50 h.
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