ArticlePloS one2011
Mitochondrial structure and function are disrupted by standard isolation methods.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 166 papers.
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Who cites it
166 citing papers in PubMed, 312 citations in OpenAlex.
- Citius, Maius, Melius: Mitochondria and Endurance Performance.Sports medicine (Auckland, N.Z.) · 2026Review
- Investigating Mitochondrial Bioenergetics in Intact Heart and Brain Tissue Slices.Current protocols · 2026Article
- The metabokine β-aminoisobutyric acid mediates exercise performance and skeletal muscle adaptation through a PGC1α-BAIBA-PPARδ axis.Nature communications · 2026Article
- Light-Activated Isolation of High-Quality Mitochondria for Therapeutic Transplantation.Angewandte Chemie (International ed. in English) · 2026Article
- Postmortem energy metabolism and meat quality development: Advances in basic pathways, endogenous regulatory factors, and exogenous management strategies.Journal of advanced research · 2026Review
- Mitophagy in Hepatic Ischemia-Reperfusion Injury: From Mitochondrial Dysfunction to Therapeutic Targeting.Biomolecules · 2026Review
- In vivo quantification of mitochondrial OThe Journal of physiology · 2026Article
- Muscle mitochondria, function, mass, and quality of life in prostate cancer during androgen deprivation therapy.Nature communications · 2026Article
- Bridging nanomechanics and bioenergetics of single mitochondria by atomic force microscopy.Life science alliance · 2026Article
- Dietary nitrate and resveratrol co-supplementation prevent HFD-mediated impairments in carotid blood flow and behavioral parameters in male mice.Physiological reports · 2026Article
- Quantification of Mitochondrial Oxidative Phosphorylation in Skeletal Muscle.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Bioenergetic profiles and respiratory control in mitochondrial physiology: Precision analysis of oxidative phosphorylation.Experimental physiology · 2026Article
- Rethinking mitochondrial isolation for the next generation of mitochondrial therapies.Frontiers in physiology · 2026Review
- β-Cell Mitochondrial Dysfunction: Underlying Mechanisms and Potential Therapeutic Strategies.Cells · 2025Review
- Chamber oxygen concentration impacts mitochondrial function and hydrogen peroxide appearance in permeabilized human skeletal muscle fibers.Biochimica et biophysica acta. Bioenergetics · 2025Article
- Efficient identification of new small molecules targeting succinate dehydrogenase in non-small cell lung cancer.Cancer cell international · 2025Article
- α-Parvin Promotes Glucose Uptake and Metabolism in Skeletal Muscle with Minimal Influence on Hepatic Insulin Sensitivity.bioRxiv : the preprint server for biology · 2025Article
- Biological sex does not impact intrinsic mitochondrial respiration supported by complexes I and II in human skeletal muscle.Experimental physiology · 2025Article
- Sex-specific decline in prefrontal cortex mitochondrial bioenergetics in aging baboons correlates with walking speed.Neurobiology of aging · 2025Article
- Review
106 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Mitochondria regulate critical components of cellular function via ATP production, reactive oxygen species production, Ca(2+) handling and apoptotic signaling. Two classical methods exist to study mitochondrial function of skeletal muscles: isolated mitochondria and permeabilized myofibers. Whereas mitochondrial isolation removes a portion of the mitochondria from their cellular environment, myofiber permeabilization preserves mitochondrial morphology and functional interactions with other intracellular components. Despite this, isolated mitochondria remain the most commonly used method to infer in vivo mitochondrial function. In this study, we directly compared measures of several key aspects of mitochondrial function in both isolated mitochondria and permeabilized myofibers of rat gastrocnemius muscle. Here we show that mitochondrial isolation i) induced fragmented organelle morphology; ii) dramatically sensitized the permeability transition pore sensitivity to a Ca(2+) challenge; iii) differentially altered mitochondrial respiration depending upon the respiratory conditions; and iv) dramatically increased H(2)O(2) production. These alterations are qualitatively similar to the changes in mitochondrial structure and function observed in vivo after cellular stress-induced mitochondrial fragmentation, but are generally of much greater magnitude. Furthermore, mitochondrial isolation markedly altered electron transport chain protein stoichiometry. Collectively, our results demonstrate that isolated mitochondria possess functional characteristics that differ fundamentally from those of intact mitochondria in permeabilized myofibers. Our work and that of others underscores the importance of studying mitochondrial function in tissue preparations where mitochondrial structure is preserved and all mitochondria are represented.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.