ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2011
Role of cysteine residues in cell surface expression of the human riboflavin transporter-2 (hRFT2) in intestinal epithelial cells.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Role of riboflavin deficiency in malaria pathophysiology.PLoS pathogens · 2024Review
- Promotion of rs3746804 (p. L267P) polymorphism to intracellular SLC52A3a trafficking and riboflavin transportation in esophageal cancer cells.Amino acids · 2021Article
- Lumiflavin increases the sensitivity of ovarian cancer stem-like cells to cisplatin by interfering with riboflavin.Journal of cellular and molecular medicine · 2019Article
- Identification of transmembrane protein 237 as a novel interactor with the intestinal riboflavin transporter-3 (RFVT-3): role in functionality and cell biology.American journal of physiology. Cell physiology · 2019Article
- Role of MicroRNA-423-5p in posttranscriptional regulation of the intestinal riboflavin transporter-3.American journal of physiology. Gastrointestinal and liver physiology · 2017Article
- Structure/functional aspects of the human riboflavin transporter-3 (American journal of physiology. Cell physiology · 2017Article
- SLC52A2 [p.P141T] and SLC52A3 [p.N21S] causing Brown-Vialetto-Van Laere Syndrome in an Indian patient: First genetically proven case with mutations in two riboflavin transporters.Clinica chimica acta; international journal of clinical chemistry · 2016Article
- SLC52A3, A Brown-Vialetto-van Laere syndrome candidate gene is essential for mouse development, but dispensable for motor neuron differentiation.Human molecular genetics · 2016Article
- Identification and characterization of the minimal 5'-regulatory region of the human riboflavin transporter-3 (SLC52A3) in intestinal epithelial cells.American journal of physiology. Cell physiology · 2015Article
- Identification of residues/sequences in the human riboflavin transporter-2 that is important for function and cell biology.Nutrition & metabolism · 2015Article
- Nutrient transport in the mammary gland: calcium, trace minerals and water soluble vitamins.Journal of mammary gland biology and neoplasia · 2014Review
- Recent advances in transport of water-soluble vitamins in organs of the digestive system: a focus on the colon and the pancreas.American journal of physiology. Gastrointestinal and liver physiology · 2013Review
- Chronic alcohol feeding inhibits physiological and molecular parameters of intestinal and renal riboflavin transport.American journal of physiology. Cell physiology · 2013Article
- Effect of clinical mutations on functionality of the human riboflavin transporter-2 (hRFT-2).Molecular genetics and metabolism · 2012Article
- Differential expression of human riboflavin transporters -1, -2, and -3 in polarized epithelia: a key role for hRFT-2 in intestinal riboflavin uptake.Biochimica et biophysica acta · 2011Article
- The ABCs of membrane transporters in health and disease (SLC series): introduction.Molecular aspects of medicineReview
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
The water-soluble vitamin B2 (riboflavin, RF) is an essential micronutrient for normal cell function and survival. Recent studies have identified a role for the human riboflavin transporter-2 (hRFT2) in normal intestinal RF absorption. However, little is known about the cell biology of this transporter and specifically about the molecular determinant(s) that dictate its cell surface expression in human intestinal epithelial cells. Here we show that the full-length hRFT2 protein fused to green fluorescent protein (GFP) (GFP-hRFT2) is expressed exclusively at the apical membrane domain of Caco-2 cells. COOH-terminal sequence was essential in dictating cell surface expression with a specific role for conserved cysteine residues (C463 and C467). Mutation of C463 and C467 ablated RF uptake, explained by retention of the constructs within the endoplasmic reticulum. Modeling analysis suggested a potential disulfide bridge between C463 and C386. Consistent with this prediction, mutating the C386 site in the context of the full-length transporter resulted in intracellular retention, whereas mutation of another conserved cysteine (C326A) was without effect on hRFT2 targeting. Intracellular trafficking of hRFT2 was also examined and appeared to involve distinct vesicular structures, the motility of vesicles critically dependent on an intact microtubule network. These results demonstrate a potential role for specific cysteine residues in the cell surface expression of the hRFT2 in human intestinal epithelial cells.
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