ArticlePlacenta2011
Oxygen levels that optimize TSC culture are identified by maximizing growth rates and minimizing stress.
Article in Placenta, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 40 citations in OpenAlex.
- The integrated developmental stress response (ISR-DASR) shift reveals a functional transition from cellular to stem cell organismal adaptation.Scientific reports · 2026Article
- Efficient stem cell-derived mouse embryo models for environmental studies.Developmental cell · 2026Article
- Stress Factors as Possible Regulators of Pluripotent Stem Cell Survival and Differentiation.Biology · 2023Review
- Using high throughput screens to predict miscarriages with placental stem cells and long-term stress effects with embryonic stem cells.Birth defects research · 2022Review
- Using Live Imaging and Fluorescence Ubiquitinated Cell Cycle Indicator Embryonic Stem Cells to Distinguish G1 Cell Cycle Delays for General Stressors like Perfluoro-Octanoic Acid and Hyperosmotic Sorbitol or G2 Cell Cycle Delay for Mutagenic Stressors like Benzo(a)pyrene.Stem cells and development · 2022Article
- Using Live Imaging and FUCCI Embryonic Stem Cells to Rank DevTox Risks: Adverse Growth Effects of PFOA Compared With DEP Are 26 Times Faster, 1,000 Times More Sensitive, and 13 Times Greater in Magnitude.Frontiers in toxicology · 2021Article
- Establishment of an in vitro placental barrier model cultured under physiologically relevant oxygen levels.Molecular human reproduction · 2020Article
- Stress Forces First Lineage Differentiation of Mouse Embryonic Stem Cells; Validation of a High-Throughput Screen for Toxicant Stress.Stem cells and development · 2019Article
- Effects of Gravity, Microgravity or Microgravity Simulation on Early Mammalian Development.Stem cells and development · 2018Review
- Hypoxia and Placental Development.Birth defects research · 2017Review
- Using stem cell oxygen physiology to optimize blastocyst culture while minimizing hypoxic stress.Journal of assisted reproduction and genetics · 2017Article
- Blastocyst-Derived Stem Cell Populations under Stress: Impact of Nutrition and Metabolism on Stem Cell Potency Loss and Miscarriage.Stem cell reviews and reports · 2017Review
- Departure from optimal OThe Journal of reproduction and development · 2017Article
- HIF-KDM3A-MMP12 regulatory circuit ensures trophoblast plasticity and placental adaptations to hypoxia.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Hypoxic Stress Forces Irreversible Differentiation of a Majority of Mouse Trophoblast Stem Cells Despite FGF4.Biology of reproduction · 2016Article
- Commonly used fertility drugs, a diet supplement, and stress force AMPK-dependent block of stemness and development in cultured mammalian embryos.Journal of assisted reproduction and genetics · 2016Article
- Comparison of 2, 5, and 20 % O2 on the development of post-thaw human embryos.Journal of assisted reproduction and genetics · 2016Article
- Stress-induced enzyme activation primes murine embryonic stem cells to differentiate toward the first extraembryonic lineage.Stem cells and development · 2014Article
- Hypoxic stress induces, but cannot sustain trophoblast stem cell differentiation to labyrinthine placenta due to mitochondrial insufficiency.Stem cell research · 2014Article
- Adaptive mechanisms controlling uterine spiral artery remodeling during the establishment of pregnancy.The International journal of developmental biology · 2014Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Accumulating data suggest that 20% O(2) causes human and mouse placental trophoblast stem cell (TSC) differentiation and suppresses proliferation. We tested the hypotheses that phosphorylated stress-activated protein kinase (pSAPK) levels report the optimal O(2) level for TSC culture, and that pSAPK responds to contradictory signals. We tested the dose range of 0-20% O(2) (0, 0.5, 2, and 20%) on five effects in cultured TSC. The results showed 1) TSC accumulation rates were highest at 2% O(2), lower at 20% and lowest at 0-0.5%; 2) pSAPK protein levels were lowest at 2% O(2), higher at 20%, and highest at 0-0.5%; 3) Cleaved caspase 3, an apoptosis marker, increased at 0.5% O(2), and was highest at 0% O(2); 4) Three markers for multipotency were highest at 2 and 20% and significantly decreased at 0.5%-0%; 5) In contrast three differentiation markers were lowest at 2% and highest at 0.5%-0%. Thus, 2% O(2) is the optimum as defined by lowest pSAPK and differentiation markers and highest growth rate and multipotency markers, without appreciable apoptosis. In addition, two lines of evidence suggest that fibroblast growth factor (FGF)4 does not directly activate SAPK. SAPK activity increases transiently with FGF4 removal at 2% O(2), but SAPK activity decreases when O(2) is switched from 20% to 2% with FGF4 present. Thus, SAPK is activated by contradictory signals, but activity decreases when either signal is removed. Taken together, the findings suggest that pSAPK senses suboptimal signals during TSC culture and probably in vivo.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.