ArticleJournal of clinical microbiology2011
Evaluation and clinical validation of an alcohol-based transport medium for preservation and inactivation of respiratory viruses.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- Practical Guidance for Clinical Microbiology Laboratories: Viruses Causing Acute Respiratory Tract Infections.Clinical microbiology reviews · 2019Guideline
- Comparison of asymptomatic and symptomatic rhinovirus infections in university students: incidence, species diversity, and viral load.Diagnostic microbiology and infectious disease · 2015Trial
- Vitamin D3 and gargling for the prevention of upper respiratory tract infections: a randomized controlled trial.BMC infectious diseases · 2014Trial
- Modified transport medium for improving influenza virus detection.Frontiers in cellular and infection microbiology · 2024Article
- Inactivation of highly transmissible livestock and avian viruses including influenza A and Newcastle disease virus for molecular diagnostics.Frontiers in veterinary science · 2024Article
- From cold chain to ambient temperature: transport of viral specimens- a review.Annals of medicine · 2023Review
- Consolidating the potency of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) in viral diagnosis: Extrapolating its applicability for COVID diagnosis?Trends in analytical chemistry : TRAC · 2022Review
- Evaluation and Clinical Validation of Guanidine-Based Inactivation Transport Medium for Preservation of SARS-CoV-2.Advances in pharmacological and pharmaceutical sciences · 2022Article
- Article
- Approach to Assessment of New Swabs and Viral Transport Media for SARS-CoV-2 Testing.Journal of clinical microbiology · 2021Article
- Comparison analysis of different swabs and transport mediums suitable for SARS-CoV-2 testing following shortages.Journal of virological methods · 2020Article
- Feasibility of a birth cohort study dedicated to assessing acute infections using symptom diaries and parental collection of biomaterials.BMC infectious diseases · 2015Article
- Rapid and generic identification of influenza A and other respiratory viruses with mass spectrometry.Journal of virological methods · 2015Article
- Alcohol consumption has a protective effect against hematological malignancies: a population-based study in Sweden including 420,489 individuals with alcohol use disorders.Neoplasia (New York, N.Y.) · 2014Article
- The common cold: potential for future prevention or cure.Current allergy and asthma reports · 2014Review
- Use of an improved quantitative polymerase chain reaction assay to determine differences in human rhinovirus viral loads in different populations.Diagnostic microbiology and infectious disease · 2012Article
- Equivalence of self- and staff-collected nasal swabs for the detection of viral respiratory pathogens.PloS one · 2012Article
- E-mail-based symptomatic surveillance combined with self-collection of nasal swabs: a new tool for acute respiratory infection epidemiology.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2011Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
The clinical and public health importance of influenza and other respiratory viruses has accelerated the development of highly sensitive molecular diagnostics, but data are limited regarding preanalytical stages of diagnostic testing. We evaluated CyMol, an alcohol-based transport medium, for its ability to maintain specimen integrity for up to 21 days of storage at various temperatures; for its ability to inactivate virus; and for its compatibility with antigen- or nucleic acid-based diagnostics for respiratory viruses in clinical samples. In mock-infected samples, both universal transport medium (UTM-RT) and CyMol maintained equivalent viral quantities for at least 14 days at room temperature or colder, whereas a dry swab collection maintained viral quantities only if refrigerated or frozen. CyMol inactivated influenza virus within 5 min of sample immersion. UTM-RT- and CyMol-collected nasal swab specimens from 73 symptomatic students attending a campus health clinic were positive for a respiratory virus in 56.2% of subjects by multiplex PCR testing, including influenza A and B viruses, rhinovirus/enteroviruses, coronaviruses, respiratory syncytial virus, parainfluenza viruses, metapneumovirus, and adenovirus. Detection by PCR was equivalent in UTM-RT- and CyMol-collected specimens and in self- and staff-collected swabs. Direct fluorescent antibody (DFA) testing was substantially less sensitive (23.3%) than multiplex PCR, and DFA testing from UTM-RT-collected swabs was more sensitive than that from CyMol-collected swabs. These data indicate that an alcohol-based transport medium such as CyMol preserves respiratory virus integrity, rapidly inactivates viruses, and is compatible with PCR-based respiratory diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.