ArticleJournal of clinical microbiology2011
Cytochrome b gene quantitative PCR for diagnosing Plasmodium falciparum infection in travelers.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- Common Molecular Detection of the Neglected Human Malaria Parasite Among Febrile Patients in Southern Regions in Senegal.Pathogens (Basel, Switzerland) · 2025Article
- Performance of a novel melting curve-based qPCR assay for malaria parasites in routine clinical practice in non-endemic setting.Malaria journal · 2023Article
- Gene drive mosquitoes can aid malaria elimination by retardingScience advances · 2022Article
- Evaluation of a novel real-time PCR assay for the detection, identification and quantification of Plasmodium species causing malaria in humans.Malaria journal · 2021Article
- An Optimized Real-Time qPCR Method for the Effective Detection of Human Malaria Infections.Diagnostics (Basel, Switzerland) · 2021Article
- Development and Clinical Validation of Iso-IMRS: A Novel Diagnostic Assay forAnalytical chemistry · 2021Article
- Ultrasensitive CRISPR-based diagnostic for field-applicable detection ofProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Template copy number and the sensitivity of quantitative PCR for Plasmodium falciparum in asymptomatic individuals.Malaria journal · 2020Article
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- Assessment of malaria real-time PCR methods and application with focus on low-level parasitaemia.PloS one · 2019Article
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- Development of a rapid HRM qPCR for the diagnosis of the four most prevalent Plasmodium lineages in New Zealand.Parasitology research · 2017Article
- Comparison of methods for detecting asymptomatic malaria infections in the China-Myanmar border area.Malaria journal · 2017Article
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- Article
- Human malaria diagnosis using a single-step direct-PCR based on the Plasmodium cytochrome oxidase III gene.Malaria journal · 2016Article
- Differential Diagnosis of Malaria on Truelab Uno®, a Portable, Real-Time, MicroPCR Device for Point-Of-Care Applications.PloS one · 2016Article
- Ultra-sensitive detection of Plasmodium falciparum by amplification of multi-copy subtelomeric targets.PLoS medicine · 2015Article
- Article
- High-throughput ultrasensitive molecular techniques for quantifying low-density malaria parasitemias.Journal of clinical microbiology · 2014Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cytochrome b (cytb) gene quantitative PCR (qPCR) assay was developed to diagnose malaria in travelers. First, manual and automated DNA extractions were compared and automated DNA extraction of 400 μl of blood was found to be more efficient. Sensitivity was estimated using the WHO international standard for Plasmodium falciparum DNA and compared to that of a previously published qPCR targeting the 18S rRNA coding gene (18S qPCR). The limit of detection of the cytb qPCR assay was 20 DNA copies (i.e., 1 parasite equivalent) per 400 μl of extracted whole blood and was comparable for the two qPCR assays. Both qPCR assays were used on blood samples from 265 consecutive patients seen for suspicion of malaria. There were no microscopy-positive and qPCR-negative samples. Positive cytb qPCR results were observed for 51 samples, and all but 1 were also 18S qPCR positive. Eight (16%) of these 51 samples were negative by microscopic examination. The 8 cytb qPCR-positive and microscopy-negative samples were from African patients, 3 of whom had received antimalarial drugs. Three non-P. falciparum infections were correctly identified using an additional qPCR assay. The absence of PCR inhibitors was tested for by the use of an internal control of mouse DNA to allow reliable quantification of circulating DNA. The high analytical sensitivity of both qPCR assays combined with automated DNA extraction supports its use as a laboratory tool for diagnosis and parasitemia determination in emergencies. Whether to treat qPCR-positive and microscopy-negative patients remains to be determined.
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