ArticleThe Journal of biological chemistry2011
A single zinc ion is sufficient for an active Trypanosoma brucei tRNA editing deaminase.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- ADAT3 variants disrupt the activity of the ADAT tRNA deaminase complex and impair neuronal migration.Brain : a journal of neurology · 2025Article
- Structural basis for sequence-independent substrate selection by eukaryotic wobble base tRNA deaminase ADAT2/3.Nature communications · 2022Article
- Identification and rescue of a tRNA wobble inosine deficiency causing intellectual disability disorder.RNA (New York, N.Y.) · 2020Article
- A plant pentatricopeptide repeat protein with a DYW-deaminase domain is sufficient for catalyzing C-to-U RNA editingThe Journal of biological chemistry · 2020Article
- Formation of tRNA Wobble Inosine in Humans Is Disrupted by a Millennia-Old Mutation Causing Intellectual Disability.Molecular and cellular biology · 2019Article
- tRNA deamination by ADAT requires substrate-specific recognition mechanisms and can be inhibited by tRFs.RNA (New York, N.Y.) · 2019Article
- A protein-protein interaction underlies the molecular basis for substrate recognition by an adenosine-to-inosine RNA-editing enzyme.Nucleic acids research · 2018Article
- From Prebiotics to Probiotics: The Evolution and Functions of tRNA Modifications.Life (Basel, Switzerland) · 2016Review
- Distribution of ADAT-Dependent Codons in the Human Transcriptome.International journal of molecular sciences · 2015Article
- A conserved glutamate residue in the C-terminal deaminase domain of pentatricopeptide repeat proteins is required for RNA editing activity.The Journal of biological chemistry · 2015Article
- Two-subunit enzymes involved in eukaryotic post-transcriptional tRNA modification.RNA biology · 2014Review
- The T. brucei TRM5 methyltransferase plays an essential role in mitochondrial protein synthesis and function.RNA (New York, N.Y.) · 2013Article
- Determinants of tRNA editing and modification: avoiding conundrums, affecting function.Seminars in cell & developmental biology · 2012Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Editing of adenosine (A) to inosine (I) at the first anticodon position in tRNA is catalyzed by adenosine deaminases acting on tRNA (ADATs). This essential reaction in bacteria and eukarya permits a single tRNA to decode multiple codons. Bacterial ADATa is a homodimer with two bound essential Zn(2+). The ADATa crystal structure revealed residues important for substrate binding and catalysis; however, such high resolution structural information is not available for eukaryotic tRNA deaminases. Despite significant sequence similarity among deaminases, we continue to uncover unexpected functional differences between Trypanosoma brucei ADAT2/3 (TbADAT2/3) and its bacterial counterpart. Previously, we demonstrated that TbADAT2/3 is unique in catalyzing two different deamination reactions. Here we show by kinetic analyses and inductively coupled plasma emission spectrometry that wild type TbADAT2/3 coordinates two Zn(2+) per heterodimer, but unlike any other tRNA deaminase, mutation of one of the key Zn(2+)-coordinating cysteines in TbADAT2 yields a functional enzyme with a single-bound zinc. These data suggest that, at least, TbADAT3 may play a role in catalysis via direct coordination of the catalytic Zn(2+). These observations raise the possibility of an unusual Zn(2+) coordination interface with important implications for the function and evolution of editing deaminases.
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Registered trials
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