ArticleThe Journal of biological chemistry2011
Gephyrin regulates GABAergic and glutamatergic synaptic transmission in hippocampal cell cultures.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 27 citations in OpenAlex.
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- Genetically encoded intrabodies as high-precision tools to visualize and manipulate neuronal function.Seminars in cell & developmental biology · 2022Article
- The Yin and Yang of GABAergic and Glutamatergic Synaptic Plasticity: Opposites in Balance by Crosstalking Mechanisms.Frontiers in synaptic neuroscience · 2022Review
- GABAergic System Dysfunction in Autism Spectrum Disorders.Frontiers in cell and developmental biology · 2021Review
- Recombinant Antibodies in Basic Neuroscience Research.Current protocols in neuroscience · 2020Review
- Tuning GABAergic Inhibition: Gephyrin Molecular Organization and Functions.Neuroscience · 2020Review
- Computational Modeling of Inhibitory Transsynaptic Signaling in Hippocampal and Cortical Neurons Expressing Intrabodies Against Gephyrin.Frontiers in cellular neuroscience · 2020Article
- An E3-ligase-based method for ablating inhibitory synapses.Nature methods · 2016Article
- Matrix Metalloproteinase-9 Regulates Neuronal Circuit Development and Excitability.Molecular neurobiology · 2016Article
- Role of primary afferents in the developmental regulation of motor axon synapse numbers on Renshaw cells.The Journal of comparative neurology · 2016Article
- A General Procedure to Study Subcellular Models of Transsynaptic Signaling at Inhibitory Synapses.Frontiers in neuroinformatics · 2016Article
- Correlation between hippocampal sulcus width and severity of obstructive sleep apnea syndrome.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2015Article
- Specific GFP-binding artificial proteins (αRep): a new tool for in vitro to live cell applications.Bioscience reports · 2015Article
- Pin1-dependent signalling negatively affects GABAergic transmission by modulating neuroligin2/gephyrin interaction.Nature communications · 2014Article
- Diffusion dynamics of synaptic molecules during inhibitory postsynaptic plasticity.Frontiers in cellular neuroscience · 2014Review
- Genetic insights into the functional elements of language.Human genetics · 2013Review
- Article
- A matter of balance: role of neurexin and neuroligin at the synapse.Neurochemical research · 2013Review
- Developmental regulation of GABAergic signalling in the hippocampus of neuroligin 3 R451C knock-in mice: an animal model of Autism.Frontiers in cellular neuroscience · 2013Article
- Gephyrin, the enigmatic organizer at GABAergic synapses.Frontiers in cellular neuroscience · 2012Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gephyrin is a scaffold protein essential for stabilizing glycine and GABA(A) receptors at inhibitory synapses. Here, recombinant intrabodies against gephyrin (scFv-gephyrin) were used to assess whether this protein exerts a transynaptic action on GABA and glutamate release. Pair recordings from interconnected hippocampal cells in culture revealed a reduced probability of GABA release in scFv-gephyrin-transfected neurons compared with controls. This effect was associated with a significant decrease in VGAT, the vesicular GABA transporter, and in neuroligin 2 (NLG2), a protein that, interacting with neurexins, ensures the cross-talk between the post- and presynaptic sites. Interestingly, hampering gephyrin function also produced a significant reduction in VGLUT, the vesicular glutamate transporter, an effect accompanied by a significant decrease in frequency of miniature excitatory postsynaptic currents. Overexpressing NLG2 in gephyrin-deprived neurons rescued GABAergic but not glutamatergic innervation, suggesting that the observed changes in the latter were not due to a homeostatic compensatory mechanism. Pulldown experiments demonstrated that gephyrin interacts not only with NLG2 but also with NLG1, the isoform enriched at excitatory synapses. These results suggest a key role of gephyrin in regulating transynaptic signaling at both inhibitory and excitatory synapses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.