ArticleGut pathogens2011
PCR-based genotyping of Helicobacter pylori of Gambian children and adults directly from biopsy specimens and bacterial cultures.
Article in Gut pathogens, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The trial behind it
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Who cites it
5 citing papers in PubMed, 24 citations in OpenAlex.
- Review
- GENETIC MUTATIONS AFFECTING THE FIRST LINE ERADICATION THERAPY OF Helicobacter pylori-INFECTED EGYPTIAN PATIENTS.Revista do Instituto de Medicina Tropical de Sao Paulo · 2016Article
- Population genetic analyses of Helicobacter pylori isolates from Gambian adults and children.PloS one · 2014Article
- Antimicrobial susceptibility and resistance patterns among Helicobacter pylori strains from The Gambia, West Africa.Antimicrobial agents and chemotherapy · 2013Article
- Mixed infection with cagA positive and cagA negative strains of Helicobacter pylori lowers disease burden in The Gambia.PloS one · 2011Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
backgroundHelicobacter pylori is an important agent of gastroduodenal disease in Africa and throughout the world. We sought to determine an optimum method for genotyping H. pylori strains from children and adults in The Gambia, West Africa.
resultsVirulence genes were amplified in 127 of 190 cases tested (121 adults and 6 children); each of 60 bacterial cultures, and 116 from DNA extracted directly from biopsies. The proportion of biopsies that were cagA+, the ratio of vacAs1/s2, and vacAm1/m2, and the proportion of mixed strain populations in individual subjects changed with age. Strains lacking virulence cagA and vacA genes and with apparently homogeneous (one predominant strain) infections were more common among infants than adults.
conclusionsIn order to detect the range of bacterial genotypes harbored by individual patients, direct PCR proved slightly superior to isolation of H. pylori by biopsy culture, but the techniques were complementary, and the combination of both culture and direct PCR produced the most complete picture. The seemingly higher virulence of strains from adult than infant infections in The Gambia merits further analysis.
Identifiers
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Registered trials
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