ArticleCancer microenvironment : official journal of the International Cancer Microenvironment Society2010
Microdialysis combined with proteomics for protein identification in breast tumor microenvironment in vivo.
Article in Cancer microenvironment : official journal of the International Cancer Microenvironment Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- Novel application of in vivo microdialysis in a rat Achilles tendon acute injury model.Journal of applied physiology (Bethesda, Md. : 1985) · 2024Article
- Alpha-smooth muscle actin-positive cancer-associated fibroblasts secreting osteopontin promote growth of luminal breast cancer.Cellular & molecular biology letters · 2022Article
- Asiaticoside ameliorates β-amyloid-induced learning and memory deficits in rats by inhibiting mitochondrial apoptosis and reducing inflammatory factors.Experimental and therapeutic medicine · 2017Article
- The Angiogenic Secretome in VEGF overexpressing Breast Cancer Xenografts.Scientific reports · 2016Article
- The fibroblast Tiam1-osteopontin pathway modulates breast cancer invasion and metastasis.Breast cancer research : BCR · 2016Article
- Tumor Interstitial Fluid Formation, Characterization, and Clinical Implications.Frontiers in oncology · 2015Review
- In vivo microdialysis sampling of adipokines CCL2, IL-6, and leptin in the mammary fat pad of adult female rats.Molecular bioSystems · 2014Article
- Advances in the proteomic investigation of the cell secretome.Expert review of proteomics · 2012Review
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
unlabelledTumor microenvironment constitutes a reservoir for proteins released from tumor cells and the host, which can contribute significantly to tumor growth and invasion. This study aims to apply a method of combining in vivo microdialysis and proteomics to identify proteins in mammary tumor interstitial fluids, a major component of tumor microenvironment. In vivo microdialysis was performed in polyomavirus middle T antigen (PyVmT) transgenic mouse mammary tumors and age-matched control wild-type mammary glands. Over four hundred proteins were identified from the microdialysis perfusates, using the Multidimensional Protein Identification Technology. Osteopontin (OPN) is one of the proteins overexpressed in breast tumor perfusates, as confirmed with immunoassays. OPN was also found to be present in tumor-associated stroma in both PyVmT and human breast tumors, using immunohistochemistry. Specifically, fibroblasts were further shown to express OPN at both mRNA and protein levels. In vitro assays showed that OPN can stimulate PyVmT breast carcinoma cell proliferation and migration. Finally, the expression of OPN was significantly higher in the peripheral blood of mice bearing breast tumors, compared to wild-type mice. Overall, microdialysis combined with proteomics is a unique technique for identifying proteins in a tumor microenvironment in vivo. Mammary fibroblasts can secrete OPN, and its overexpression in mammary tumor microenvironment may contribute significantly to mammary tumor progression. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12307-010-0046-3) contains supplementary material, which is available to authorized users.
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