ArticleThe EMBO journal2011
Conjugal plasmid transfer in Streptomyces resembles bacterial chromosome segregation by FtsK/SpoIIIE.
Article in The EMBO journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 69 citations in OpenAlex.
- Giant linear plasmids in Mycobacterium avium harbour a tRNA array unit.DNA research : an international journal for rapid publication of reports on genes and genomes · 2026Article
- Type IV secretion systems: reconciling diversity through a unified nomenclature.FEMS microbiology reviews · 2026Review
- The transcriptomic response of Staphylococcus equorum KS1030 to Lincomycin stress reveals transporters associated with horizontal gene transfer.Scientific reports · 2025Article
- Conjugation Mediates Large-Scale Chromosomal Transfer in Streptomyces Driving Diversification of Antibiotic Biosynthetic Gene Clusters.Molecular biology and evolution · 2024Article
- Identification of plasmids from thermophilic Streptomyces strains and development of a gene cloning system for thermophilic Streptomyces species.Journal of industrial microbiology & biotechnology · 2024Article
- Revolving ATPase motors as asymmetrical hexamers in translocating lengthy dsDNA via conformational changes and electrostatic interactions in phi29, T7, herpesvirus, mimivirus,Exploration (Beijing, China) · 2023Review
- Unravelling the DNA sequences carried by Streptomyces coelicolor membrane vesicles.Scientific reports · 2022Article
- Prevalence and mobility of integrative and conjugative elements within aFrontiers in microbiology · 2022Article
- Chromosome Segregation Proteins as Coordinators of Cell Cycle in Response to Environmental Conditions.Frontiers in microbiology · 2020Review
- Highlights of Streptomyces genetics.Heredity · 2019Review
- The pathogenic actinobacterium Rhodococcus equi: what's in a name?Molecular microbiology · 2019Review
- The FtsK-like motor TraB is a DNA-dependent ATPase that forms higher-order assemblies.The Journal of biological chemistry · 2019Article
- Blending genomes: distributive conjugal transfer in mycobacteria, a sexier form of HGT.Molecular microbiology · 2018Review
- The transjugation machinery of Thermus thermophilus: Identification of TdtA, an ATPase involved in DNA donation.PLoS genetics · 2017Article
- An Invertron-Like Linear Plasmid Mediates Intracellular Survival and Virulence in Bovine Isolates of Rhodococcus equi.Infection and immunity · 2015Article
- Article
- Noncanonical cell-to-cell DNA transfer in Thermus spp. is insensitive to argonaute-mediated interference.Journal of bacteriology · 2015Article
- Distributive Conjugal Transfer: New Insights into Horizontal Gene Transfer and Genetic Exchange in Mycobacteria.Microbiology spectrum · 2014Article
- Social behavior and decision making in bacterial conjugation.Frontiers in cellular and infection microbiology · 2014Review
- The stability region of the Streptomyces lividans plasmid pIJ101 encodes a DNA-binding protein recognizing a highly conserved short palindromic sequence motif.Frontiers in microbiology · 2014Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conjugation is a major route of horizontal gene transfer, the driving force in the evolution of bacterial genomes. Antibiotic producing soil bacteria of the genus Streptomyces transfer DNA in a unique process involving a single plasmid-encoded protein TraB and a double-stranded DNA molecule. However, the molecular function of TraB in directing DNA transfer from a donor into a recipient cell is unknown. Here, we show that TraB constitutes a novel conjugation system that is clearly distinguished from DNA transfer by a type IV secretion system. We demonstrate that TraB specifically recognizes and binds to repeated 8 bp motifs on the conjugative plasmid. The specific DNA recognition is mediated by helix α3 of the C-terminal winged-helix-turn-helix domain of TraB. We show that TraB assembles to a hexameric ring structure with a central ∼3.1 nm channel and forms pores in lipid bilayers. Structure, sequence similarity and DNA binding characteristics of TraB indicate that TraB is derived from an FtsK-like ancestor protein, suggesting that Streptomyces adapted the FtsK/SpoIIIE chromosome segregation system to transfer DNA between two distinct Streptomyces cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.