ArticleAmerican journal of physiology. Endocrinology and metabolism2011
Plasma membrane electron transport in pancreatic β-cells is mediated in part by NQO1.
Article in American journal of physiology. Endocrinology and metabolism, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
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- Predicting the outcome of Thoroughbred stallion matings on the basis of dismount semen sample analyses.Reproduction (Cambridge, England) · 2023Article
- Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumors.Science advances · 2022Article
- Benzotriazine Di-Oxide Prodrugs for Exploiting Hypoxia and Low Extracellular pH in Tumors.Molecules (Basel, Switzerland) · 2019Article
- The zinc transporter Slc39a5 controls glucose sensing and insulin secretion in pancreatic β-cells via Sirt1- and Pgc-1α-mediated regulation of Glut2.Protein & cell · 2019Article
- How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions.Oxidative medicine and cellular longevity · 2018Review
- Positive Feedback Amplifies the Response of Mitochondrial Membrane Potential to Glucose Concentration in Clonal Pancreatic Beta Cells.Biochimica et biophysica acta. Molecular basis of disease · 2017Article
- NAD(P)H-dependent quinone oxidoreductase 1 (NQO1) and cytochrome P450 oxidoreductase (CYP450OR) differentially regulate menadione-mediated alterations in redox status, survival and metabolism in pancreatic β-cells.Toxicology letters · 2016Article
- Mechanisms of Doxorubicin Toxicity in Pancreatic β-Cells.Toxicological sciences : an official journal of the Society of Toxicology · 2016Article
- Calorie restriction as an intervention in ageing.The Journal of physiology · 2016Review
- Thymoquinone, a bioactive component of Nigella sativa, normalizes insulin secretion from pancreatic β-cells under glucose overload via regulation of malonyl-CoA.American journal of physiology. Endocrinology and metabolism · 2016Article
- Sirtuin activation: a role for plasma membrane in the cell growth puzzle.The journals of gerontology. Series A, Biological sciences and medical sciences · 2013Article
- NAD kinase regulates the size of the NADPH pool and insulin secretion in pancreatic β-cells.American journal of physiology. Endocrinology and metabolism · 2012Article
- The level of menadione redox-cycling in pancreatic β-cells is proportional to the glucose concentration: role of NADH and consequences for insulin secretion.Toxicology and applied pharmacology · 2012Article
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Plasma membrane electron transport (PMET), a cytosolic/plasma membrane analog of mitochondrial electron transport, is a ubiquitous system of cytosolic and plasma membrane oxidoreductases that oxidizes cytosolic NADH and NADPH and passes electrons to extracellular targets. While PMET has been shown to play an important role in a variety of cell types, no studies exist to evaluate its function in insulin-secreting cells. Here we demonstrate the presence of robust PMET activity in primary islets and clonal β-cells, as assessed by the reduction of the plasma membrane-impermeable dyes WST-1 and ferricyanide. Because the degree of metabolic function of β-cells (reflected by the level of insulin output) increases in a glucose-dependent manner between 4 and 10 mM glucose, PMET was evaluated under these conditions. PMET activity was present at 4 mM glucose and was further stimulated at 10 mM glucose. PMET activity at 10 mM glucose was inhibited by the application of the flavoprotein inhibitor diphenylene iodonium and various antioxidants. Overexpression of cytosolic NAD(P)H-quinone oxidoreductase (NQO1) increased PMET activity in the presence of 10 mM glucose while inhibition of NQO1 by its inhibitor dicoumarol abolished this activity. Mitochondrial inhibitors rotenone, antimycin A, and potassium cyanide elevated PMET activity. Regardless of glucose levels, PMET activity was greatly enhanced by the application of aminooxyacetate, an inhibitor of the malate-aspartate shuttle. We propose a model for the role of PMET as a regulator of glycolytic flux and an important component of the metabolic machinery in β-cells.
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