ArticleMolecular and cellular biology2011
Heterochromatin is required for normal distribution of Neurospora crassa CenH3.
Article in Molecular and cellular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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77 citing papers in PubMed, 135 citations in OpenAlex.
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- Centromeres are hotspots of cytosine methylation epimutations in a filamentous fungus.bioRxiv : the preprint server for biology · 2026Article
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- CSE-8, a filamentous fungus-specific Shr3-like chaperone, facilitates endoplasmic reticulum exit of chitin synthase CHS-3 (class I) inFrontiers in fungal biology · 2024Article
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- Epigenetic regulation of nuclear processes in fungal plant pathogens.PLoS pathogens · 2023Review
- Chromatin structure influences rate and spectrum of spontaneous mutations inGenome research · 2023Article
- A highly quality genome sequence of Penicillium oxalicum species isolated from the root of Ixora chinensis in Vietnam.G3 (Bethesda, Md.) · 2023Article
- Cell cycle control of kinetochore assembly.Nucleus (Austin, Tex.) · 2022Review
- The genome organization of Neurospora crassa at high resolution uncovers principles of fungal chromosome topology.G3 (Bethesda, Md.) · 2022Article
- Differential Regulation and Production of Secondary Metabolites among Isolates of the Fungal Wheat Pathogen Zymoseptoria tritici.Applied and environmental microbiology · 2022Article
- Chromatin profiling reveals heterogeneity in clinical isolates of the human pathogen Aspergillus fumigatus.PLoS genetics · 2022Article
- The stem rust fungus Puccinia graminis f. sp. tritici induces centromeric small RNAs during late infection that are associated with genome-wide DNA methylation.BMC biology · 2021Article
- Recent insights into mechanisms preventing ectopic centromere formation.Open biology · 2021Review
- Chromosome assembled and annotated genome sequence of Aspergillus flavus NRRL 3357.G3 (Bethesda, Md.) · 2021Article
- Epigenetic dynamics of centromeres and neocentromeres in Cryptococcus deuterogattii.PLoS genetics · 2021Article
- Role of the Non-Canonical RNAi Pathway in the Antifungal Resistance and Virulence of Mucorales.Genes · 2021Review
17 more citing papers are in PubMed but not listed here.
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5 authors at 1 institution in 1 country.
Funding
Abstract
Centromeres serve as platforms for the assembly of kinetochores and are essential for nuclear division. Here we identified Neurospora crassa centromeric DNA by chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) of DNA associated with tagged versions of the centromere foundation proteins CenH3 (CENP-A) and CEN-C (CENP-C) and the kinetochore protein CEN-T (CENP-T). On each chromosome we found an ∼150- to 300-kbp region of enrichment for all three proteins. These regions correspond to intervals predicted to be centromeric DNA by genetic mapping and DNA sequence analyses. By ChIP-seq we found extensive colocalization of CenH3, CEN-C, CEN-T, and histone H3K9 trimethylation (H3K9me3). In contrast, H3K4me2, which has been found at the cores of plant, fission yeast, Drosophila, and mammalian centromeres, was not enriched in Neurospora centromeric DNA. DNA methylation was most pronounced at the periphery of centromeric DNA. Mutation of dim-5, which encodes an H3K9 methyltransferase responsible for nearly all H3K9me3, resulted in altered distribution of CenH3-green fluorescent protein (GFP). Similarly, CenH3-GFP distribution was altered in the absence of HP1, the chromodomain protein that binds to H3K9me3. We conclude that eukaryotes with regional centromeres make use of different strategies for maintenance of CenH3 at centromeres, and we suggest a model in which centromere proteins nucleate at the core but require DIM-5 and HP1 for spreading.
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