ArticleThe Journal of biological chemistry2011
The phytosulfokine (PSK) receptor is capable of guanylate cyclase activity and enabling cyclic GMP-dependent signaling in plants.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 149 citations in OpenAlex.
- A Phytosulfokine signaling module activates the Nod factor receptor to control soybean nodulation.Nature communications · 2026Article
- Biogenesis and downstream effects of 3',5' and 2',3' cAMP isomers in plants.Science advances · 2026Article
- Phosphovariants of the canonical heterotrimeric Gα protein, GPA1, differentially affect G protein activity and Arabidopsis development.bioRxiv : the preprint server for biology · 2026Article
- Guanylate cyclase activity in moss: revisiting the role of ERECTA-like receptors.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- lncRNA-Encoded Small Peptide Promotes Viral Infection.Molecular plant pathology · 2025Article
- Peptides and Reactive Oxygen Species Regulate Root Development.International journal of molecular sciences · 2025Review
- Peptide hormones in plants.Molecular horticulture · 2025Review
- "Cyclic nucleotides in plants: from obscure messengers to central regulators".Frontiers in plant science · 2025Review
- Genome-wide identification of PSKR genes in wheat and differential expression under abiotic stress conditions.Frontiers in plant science · 2025Article
- Moonlighting Crypto-Enzymes and Domains as Ancient and Versatile Signaling Devices.International journal of molecular sciences · 2024Review
- Response surface methodology mediated optimization of phytosulfokine and plant growth regulators for enhanced protoplast division, callus induction, and somatic embryogenesis in Angelica Gigas Nakai.BMC plant biology · 2024Article
- Sulfated peptides and their receptors: Key regulators of plant development and stress adaptation.Plant communications · 2024Review
- Peptide-Mediated Cyclic Nucleotide Signaling in Plants: Identification and Characterization of Interactor Proteins with Nucleotide Cyclase Activity.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Effects of drought and salt stress on the root phenotype of wheat seedlings and underlying gene expression analysis.Frontiers in plant science · 2024Article
- Sulfated peptides: key players in plant development, growth, and stress responses.Frontiers in plant science · 2024Review
- Plant adenylate cyclases have come full circle.Nature plants · 2023Review
- The Research Process of PSK Biosynthesis, Signaling Transduction, and Potential Applications inPlants (Basel, Switzerland) · 2023Review
- Ubiquitylation of PHYTOSULFOKINE RECEPTOR 1 modulates the defense response in tomato.Plant physiology · 2023Article
- A methane-cGMP module positively influences adventitious rooting.Plant cell reports · 2023Article
- Phytosulfokine peptide optimizes plant growth and defense via glutamine synthetase GS2 phosphorylation in tomato.The EMBO journal · 2023Article
8 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 3 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phytosulfokines (PSKs) are sulfated pentapeptides that stimulate plant growth and differentiation mediated by the PSK receptor (PSKR1), which is a leucine-rich repeat receptor-like kinase. We identified a putative guanylate cyclase (GC) catalytic center in PSKR1 that is embedded within the kinase domain and hypothesized that the GC works in conjunction with the kinase in downstream PSK signaling. We expressed the recombinant complete kinase (cytoplasmic) domain of AtPSKR1 and show that it has serine/threonine kinase activity using the Ser/Thr peptide 1 as a substrate with an approximate K(m) of 7.5 μm and V(max) of 1800 nmol min(-1) mg(-1) of protein. This same recombinant protein also has GC activity in vitro that is dependent on the presence of either Mg(2+) or Mn(2+). Overexpression of the full-length AtPSKR1 receptor in Arabidopsis leaf protoplasts raised the endogenous basal cGMP levels over 20-fold, indicating that the receptor has GC activity in vivo. In addition, PSK-α itself, but not the non-sulfated backbone, induces rapid increases in cGMP levels in protoplasts. Together these results indicate that the PSKR1 contains dual GC and kinase catalytic activities that operate in vivo and that this receptor constitutes a novel class of enzymes with overlapping catalytic domains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.