ArticleThe Journal of biological chemistry2011
Biochemical characterization and homology modeling of methylbutenol synthase and implications for understanding hemiterpene synthase evolution in plants.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 54 citations in OpenAlex.
- Molecular evolution of terpene synthase underlying the diversification of isoprene emission in Fagaceae.Plant biology (Stuttgart, Germany) · 2026Article
- Isopentenyl diphosphate isomerase exerts limited control over terpenoid biosynthesis in two woody plant species.Plant physiology · 2026Article
- Structural diversity of isoprene synthases in mosses from multiple terpenoid synthase lineages.The Journal of biological chemistry · 2026Article
- Cryptic isoprene emission of soybeans.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Non-enzymatic formation of isoprene and 2-methyl-3-buten-2-ol (2-MBO) by manganese.Scientific reports · 2022Article
- Monoterpene Synthase Genes and Monoterpene Profiles inPlants (Basel, Switzerland) · 2022Article
- Evolution of isoprene emission in Arecaceae (palms).Evolutionary applications · 2021Article
- On the Evolution and Functional Diversity of Terpene Synthases in the Pinus Species: A Review.Journal of molecular evolution · 2020Review
- Simultaneous Real-Time Measurement of Isoprene and 2-Methyl-3-Buten-2-ol Emissions From Trees Using SIFT-MS.Frontiers in plant science · 2020Article
- A molecular and genomic reference system for conifer defence against insects.Plant, cell & environment · 2019Review
- Isoprene: New insights into the control of emission and mediation of stress tolerance by gene expression.Plant, cell & environment · 2019Review
- Plastidic glucose-6-phosphate dehydrogenases are regulated to maintain activity in the light.The Biochemical journal · 2019Article
- In Planta Recapitulation of Isoprene Synthase Evolution from Ocimene Synthases.Molecular biology and evolution · 2017Article
- Multi-Substrate Terpene Synthases: Their Occurrence and Physiological Significance.Frontiers in plant science · 2016Review
- Molecular cloning and biochemical characterization of isoprene synthases from the tropical trees Ficus virgata, Ficus septica, and Casuarina equisetifolia.Journal of plant research · 2015Article
- Fungal Symbionts of the Spruce Bark Beetle Synthesize the Beetle Aggregation Pheromone 2-Methyl-3-buten-2-ol.Journal of chemical ecology · 2015Article
- Plant terpenes: defense responses, phylogenetic analysis, regulation and clinical applications.3 Biotech · 2015Article
- 2-Methyl-3-buten-2-ol (MBO) synthase expression in Nostoc punctiforme leads to over production of phytols.Bioengineered · 2015Article
- Article
- Bidirectional exchange of biogenic volatiles with vegetation: emission sources, reactions, breakdown and deposition.Plant, cell & environment · 2014Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
2-Methyl-3-buten-2-ol (MBO) is a five-carbon alcohol produced and emitted in large quantities by many species of pine native to western North America. MBO is structurally and biosynthetically related to isoprene and can have an important impact on regional atmospheric chemistry. The gene for MBO synthase was identified from Pinus sabiniana, and the protein encoded was functionally characterized. MBO synthase is a bifunctional enzyme that produces both MBO and isoprene in a ratio of ~90:1. Divalent cations are required for activity, whereas monovalent cations are not. MBO production is enhanced by K(+), whereas isoprene production is inhibited by K(+) such that, at physiologically relevant [K(+)], little or no isoprene emission should be detected from MBO-emitting trees. The K(m) of MBO synthase for dimethylallyl diphosphate (20 mm) is comparable with that observed for angiosperm isoprene synthases and 3 orders of magnitude higher than that observed for monoterpene and sesquiterpene synthases. Phylogenetic analysis showed that MBO synthase falls into the TPS-d1 group (gymnosperm monoterpene synthases) and is most closely related to linalool synthase from Picea abies. Structural modeling showed that up to three phenylalanine residues restrict the size of the active site and may be responsible for making this a hemiterpene synthase rather than a monoterpene synthase. One of these residues is homologous to a Phe residue found in the active site of isoprene synthases. The remaining two Phe residues do not have homologs in isoprene synthases but occupy the same space as a second Phe residue that closes off the isoprene synthase active site.
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