ArticleBiophysical journal2011
Condensation prevails over B-A transition in the structure of DNA at low humidity.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- From single DNA molecule to nanoparticle formation: mechanistic basis for monocationic aromatic drug-induced DNA frameworks.Journal of nanobiotechnology · 2026Article
- The effects of DMSO on DNA conformations and mechanics.Biophysical journal · 2025Article
- Characteristic effect of hydroxyurea on the higher-order structure of DNA and gene expression.Scientific reports · 2024Article
- Optical Tweezers to Study Viruses.Sub-cellular biochemistry · 2024Review
- Single-Molecular Dissection of Liquid-Liquid Phase Transitions.Journal of the American Chemical Society · 2023Article
- Energy Transport alongMaterials (Basel, Switzerland) · 2022Article
- Function of a viral genome packaging motor from bacteriophage T4 is insensitive to DNA sequence.Nucleic acids research · 2020Article
- Comparison of the Binding Geometry of Free-Base and Hexacoordinated Cationic Porphyrins to A- and B-Form DNA.ACS omega · 2018Article
- A DNA-centered explanation of the DNA polymerase translocation mechanism.Scientific reports · 2017Article
- Differential stability of DNA based on salt concentration.European biophysics journal : EBJ · 2017Article
- Boundary conditions for free A-DNA in solution and the relation of local to global DNA structures at reduced water activity.European biophysics journal : EBJ · 2016Article
- A Temperature-Jump Optical Trap for Single-Molecule Manipulation.Biophysical journal · 2015Article
- The scrunchworm hypothesis: transitions between A-DNA and B-DNA provide the driving force for genome packaging in double-stranded DNA bacteriophages.Journal of structural biology · 2015Article
- Optical tweezers reveal force plateau and internal friction in PEG-induced DNA condensation.European biophysics journal : EBJ · 2014Article
- Mechanistic insight into the structure and dynamics of entangled and hydrated λ-phage DNA.The journal of physical chemistry. A · 2012Article
- Mechanical properties of high-G.C content DNA with a-type base-stacking.Biophysical journal · 2011Article
Corrections and comments
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6 authors.
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Abstract
B-A transition and DNA condensation are processes regulated by base sequence and water activity. The constraints imposed by interhelical interactions in condensation compromise the observation of the mechanism by which B and A base-stacking modes influence the global state of the molecule. We used a single-molecule approach to prevent aggregation and mechanical force to control the intramolecular chain association involved in condensation. Force-extension experiments with optical tweezers revealed that DNA stretches as B-DNA under ethanol and spermine concentrations that favor the A-form. Moreover, we found no contour-length change compatible with a cooperative transition between the A and B forms within the intrinsic-force regime. Experiments performed at constant force in the entropic-force regime with magnetic tweezers similarly did not show a bistable contraction of the molecules that could be attributed to the B-A transition when the physiological buffer was replaced by a water-ethanol mixture. A total, stepwise collapse was found instead, which is characteristic of DNA condensation. Therefore, a low-humidity-induced change from the B- to the A-form base-stacking alone does not lead to a contour-length shortening. These results support a mechanism for the B-A transition in which low-humidity conditions locally change the base-stacking arrangement and globally induce DNA condensation, an effect that may eventually stabilize a molecular contour-length reduction.
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