ArticleBiophysical journal2011
In chemotaxing fibroblasts, both high-fidelity and weakly biased cell movements track the localization of PI3K signaling.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 1 citations in OpenAlex.
- A phase field model with stochastic input simulates cellular gradient sensing, morphodynamics, and fidelity of haptotaxis.PLoS computational biology · 2026Article
- Optogenetic control of PLC-γ1 activity directs cell motility.The Journal of cell biology · 2026Article
- A phase field model with stochastic input simulates cellular gradient sensing, morphodynamics, and fidelity of haptotaxis.bioRxiv : the preprint server for biology · 2026Article
- Optogenetic control of PLC-γ1 activity polarizes cell motility.bioRxiv : the preprint server for biology · 2025Article
- The mechanobiology of fibroblast activation in disease.APL bioengineering · 2025Review
- Leveraging Tunable Nanoparticle Surface Functionalization to Alter Cellular Migration.ACS nanoscience Au · 2024Article
- Article
- Microfluidic devices fitted with "flowver" paper pumps generate steady, tunable gradients for extended observation of chemotactic cell migration.Biomicrofluidics · 2021Article
- AnFrontiers in bioengineering and biotechnology · 2021Article
- Mechanistic models of PLC/PKC signaling implicate phosphatidic acid as a key amplifier of chemotactic gradient sensing.PLoS computational biology · 2020Article
- A Reaction-Diffusion Model Explains Amplification of the PLC/PKC Pathway in Fibroblast Chemotaxis.Biophysical journal · 2017Article
- Optogenetic control of RhoA reveals zyxin-mediated elasticity of stress fibres.Nature communications · 2017Article
- Quantitative Multiscale Cell Imaging in Controlled 3D Microenvironments.Developmental cell · 2016Article
- Mesenchymal chemotaxis requires selective inactivation of myosin II at the leading edge via a noncanonical PLCγ/PKCα pathway.Developmental cell · 2014Article
- Directed migration of mesenchymal cells: where signaling and the cytoskeleton meet.Current opinion in cell biology · 2014Review
- Mechanical boundary conditions bias fibroblast invasion in a collagen-fibrin wound model.Biophysical journal · 2014Article
- Migration of cells in a social context.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Fibroblast Migration Is Regulated by Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) Protein.PloS one · 2013Article
- Mechanical regulation of fibroblast migration and collagen remodelling in healing myocardial infarcts.The Journal of physiology · 2012Article
- Ca2+ pulses control local cycles of lamellipodia retraction and adhesion along the front of migrating cells.Current biology : CB · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Cell movement biased by a chemical gradient, or chemotaxis, coordinates the recruitment of cells and collective migration of cell populations. During wound healing, chemotaxis of fibroblasts is stimulated by platelet-derived growth factor (PDGF) and certain other chemoattractants. Whereas the immediate PDGF gradient sensing response has been characterized previously at the level of phosphoinositide 3-kinase (PI3K) signaling, the sensitivity of the response at the level of cell migration bias has not yet been studied quantitatively. In this work, we used live-cell total internal reflection fluorescence microscopy to monitor PI3K signaling dynamics and cell movements for extended periods. We show that persistent and properly aligned (i.e., high-fidelity) fibroblast migration does indeed correlate with polarized PI3K signaling; accordingly, this behavior is seen only under conditions of high gradient steepness (>10% across a typical cell length of 50 μm) and a certain range of PDGF concentrations. Under suboptimal conditions, cells execute a random or biased random walk, but nonetheless move in a predictable fashion according to the changing pattern of PI3K signaling. Inhibition of PI3K during chemotaxis is accompanied by loss of both cell-substratum contact and morphological polarity, but after a recovery period, PI3K-inhibited fibroblasts often regain the ability to orient toward the PDGF gradient.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.