ArticleJournal of the American Chemical Society2011
Single-molecule analysis enables free solution hydrodynamic separation using yoctomole levels of DNA.
Article in Journal of the American Chemical Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Messenger RNA and guide RNA distributions in lipid nanoparticles impact gene-editing efficiency in vivo.Molecular therapy : the journal of the American Society of Gene Therapy · 2026Article
- Single-Particle Spectroscopic Chromatography Reveals Heterogeneous RNA Loading and Size Correlations in Lipid Nanoparticles.ACS nano · 2024Article
- Payload distribution and capacity of mRNA lipid nanoparticles.Nature communications · 2022Article
- Review
- Multi-detector hydrodynamic chromatography of colloids: following in Hamish Small's footsteps.Heliyon · 2021Review
- Electrode-Free Concentration and Recovery of DNA at Physiologically Relevant Ionic Concentrations.Analytical chemistry · 2020Article
- Versatile Analysis of DNA-Biomolecule Interactions in Solution by Hydrodynamic Separation and Single Molecule Detection.Analytical chemistry · 2019Article
- Molecular rheotaxis directs DNA migration and concentration against a pressure-driven flow.Nature communications · 2017Article
- Article
- A Simple Thermoplastic Substrate Containing Hierarchical Silica Lamellae for High-Molecular-Weight DNA Extraction.Advanced materials (Deerfield Beach, Fla.) · 2016Article
- Analysis of single nucleic acid molecules in micro- and nano-fluidics.Lab on a chip · 2016Review
- Single Molecule Hydrodynamic Separation Allows Sensitive and Quantitative Analysis of DNA Conformation and Binding Interactions in Free Solution.Journal of the American Chemical Society · 2016Article
- Simultaneously sizing and quantitating zeptomole-level DNA at high throughput in free solution.Chemistry (Weinheim an der Bergstrasse, Germany) · 2014Article
- Beyond gel electrophoresis: microfluidic separations, fluorescence burst analysis, and DNA stretching.Chemical reviews · 2013Review
- An integrated, multiparametric flow cytometry chip using "microfluidic drifting" based three-dimensional hydrodynamic focusing.Biomicrofluidics · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Single-molecule free solution hydrodynamic separation (SML-FSHS) cohesively integrates cylindrical illumination confocal spectroscopy with free solution hydrodynamic separation. This technique enables single-molecule analysis of size separated DNA with 100% mass detection efficiency, high sizing resolution and wide dynamic range, surpassing the performance of single molecule capillary electrophoresis. Furthermore, SML-FSHS required only a bare fused silica microcapillary and simple pressure control rather than complex high voltage power supplies, sieving matrices, and wall coatings. The wide dynamic range and high sizing resolution of SML-FSHS was demonstrated by separating both large DNA (23 vs 27 kbp) and small DNA (100 vs 200 bp) under identical conditions. Separations were successfully performed with near zero sample consumption using as little as 5 pL of sample and 240 yoctomoles (∼150 molecules) of DNA. Quantitative accuracy was predominantly limited by molecular shot noise. Furthermore, the ability of this method to analyze of single molecule nanosensors was investigated. SML-FSHS was used to examine the thermodynamic equilibrium between stochastically open molecular beacon and target-bound molecular beacon in the detection of E. coli 16s rRNA targets.
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Registered trials
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