ArticleGenesis (New York, N.Y. : 2000)2011
A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging.
Article in Genesis (New York, N.Y. : 2000), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Trans-scale live-imaging of an E5.5 mouse embryo using incubator-type biaxial light-sheet microscopy.Life science alliance · 2025Article
- Mechanical forces across compartments coordinate cell shape and fate transitions to generate tissue architecture.Nature cell biology · 2024Article
- Spatially coordinated cell cycle activity and motility govern bifurcation of mammary branches.The Journal of cell biology · 2023Article
- SyNPL: Synthetic Notch pluripotent cell lines to monitor and manipulate cell interactions in vitro and in vivo.Development (Cambridge, England) · 2022Article
- Open-source personal pipetting robots with live-cell incubation and microscopy compatibility.Nature communications · 2022Article
- Cell influx and contractile actomyosin force drive mammary bud growth and invagination.The Journal of cell biology · 2021Article
- Expression of a membrane-targeted fluorescent reporter disrupts auditory hair cell mechanoelectrical transduction and causes profound deafness.Hearing research · 2021Article
- Experimental pathology by intravital microscopy and genetically encoded fluorescent biosensors.Pathology international · 2020Review
- Exclusive vital labeling of myonuclei for studying myonuclear arrangement in mouse skeletal muscle tissue.Skeletal muscle · 2020Article
- Dynamic organelle localization and cytoskeletal reorganization during preimplantation mouse embryo development revealed by live imaging of genetically encoded fluorescent fusion proteins.Genesis (New York, N.Y. : 2000) · 2019Article
- Synchronized mesenchymal cell polarization and differentiation shape the formation of the murine trachea and esophagus.Nature communications · 2018Article
- Spatiotemporal Labeling of Melanocytes in Mice.International journal of molecular sciences · 2018Review
- Cell Type-Specific Survey of Epigenetic Modifications by Tandem Chromatin Immunoprecipitation Sequencing.Scientific reports · 2018Article
- AAV2-Mediated Transduction of the Mouse Retina After Optic Nerve Injury.Investigative ophthalmology & visual science · 2017Article
- Imaging morphogenesis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Leader Cells Define Directionality of Trunk, but Not Cranial, Neural Crest Cell Migration.Cell reports · 2016Article
- The development and plasticity of alveolar type 1 cells.Development (Cambridge, England) · 2016Article
- Tissue-Specific Regulation of Oncogene Expression Using Cre-Inducible ROSA26 Knock-In Transgenic Mice.Current protocols in mouse biology · 2015Article
- Heterochromatin dynamics during the differentiation process revealed by the DNA methylation reporter mouse, MethylRO.Stem cell reports · 2014Article
- A Uchl1-Histone2BmCherry:GFP-gpi BAC transgene for imaging neuronal progenitors.Genesis (New York, N.Y. : 2000) · 2013Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Live-imaging is an essential tool to visualize live cells and monitor their behaviors during development. This technology demands a variety of mouse reporter lines, each uniquely expressing a fluorescent protein. Here, we developed an R26R-RG reporter mouse line that conditionally and simultaneously expresses mCherry and EGFP in nuclei and plasma membranes, respectively, from the Rosa26 locus. The intensity and resolution of mCherry nuclear localization and EGFP membrane localization were demonstrated to be sufficient for live-imaging with embryos that express RG (mCherry and EGFP) ubiquitously and specifically in fetal Sertoli cells. The conditional R26R-RG reporter mouse line should be a useful tool for labeling nuclei and membranes simultaneously in distinct cell populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.