ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2011
Recognition of Clostridium difficile PCR-ribotypes 001, 027 and 126/078 using an extended MALDI-TOF MS system.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Accelerated detection ofMicrobiology spectrum · 2026Article
- Contribution of MALDI-TOF mass spectrometry and machine learning including deep learning techniques for the detection of virulence factors of Clostridioides difficile strains.Microbial biotechnology · 2024Article
- Discrimination between hypervirulent and non-hypervirulent ribotypes of Clostridioides difficile by MALDI-TOF mass spectrometry and machine learning.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2023Article
- Current and Future Technologies for the Detection of Antibiotic-Resistant Bacteria.Diagnostics (Basel, Switzerland) · 2023Review
- Investigation of MALDI-TOF Mass Spectrometry for Assessing the Molecular Diversity ofDiagnostics (Basel, Switzerland) · 2021Article
- Bacterial Sub-Species Typing Using Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry: What Is Promising?Current issues in molecular biology · 2021Review
- MALDI-TOF MS: An alternative approach for ribotyping Clostridioides difficile isolates in Brazil.Anaerobe · 2021Article
- Rapid Classification ofMicroorganisms · 2021Article
- Transition From PCR-Ribotyping to Whole Genome Sequencing Based Typing ofFrontiers in cellular and infection microbiology · 2021Article
- Characterization ofMicroorganisms · 2020Article
- Differentiation ofEuropean journal of microbiology & immunology · 2019Article
- Proteotyping as alternate typing method to differentiate Campylobacter coli clades.Scientific reports · 2019Article
- Proteotyping ofFrontiers in microbiology · 2019Article
- Discrimination of Human Pathogen Clostridium Species Especially of the Heterogeneous C. sporogenes and C. botulinum by MALDI-TOF Mass Spectrometry.Current microbiology · 2018Article
- Identification and Characterization of Clostridium difficile Sequence Type 37 Genotype by Matrix-Assisted Laser Desorption IonizationJournal of clinical microbiology · 2018Article
- Update on Antimicrobial Resistance in Clostridium difficile: Resistance Mechanisms and Antimicrobial Susceptibility Testing.Journal of clinical microbiology · 2017Review
- Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP).Journal of visualized experiments : JoVE · 2016Article
- False Negative Results in Clostridium difficile Testing.BMC infectious diseases · 2016Article
- Clostridium difficile infection: epidemiology, diagnosis and understanding transmission.Nature reviews. Gastroenterology & hepatology · 2016Review
- Unraveling the microbiome of a thermophilic biogas plant by metagenome and metatranscriptome analysis complemented by characterization of bacterial and archaeal isolates.Biotechnology for biofuels · 2016Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
During the last decade, Clostridium difficile infection (CDI) increased markedly inside as well as outside of hospitals. In association with the occurrence of new hypervirulent C. difficile strains, CDI became more important. Until now typing of C. difficile strains has been enabled by PCR-ribotyping. However, this method is restricted to specialized laboratories combined with high maintenance cost. Therefore, we tested MALDI-TOF mass spectrometry for typing of C. difficile to provide a fast method for surveillance of CDI. Using a standard set of 25 different C. difficile PCR ribotypes a database was made by different mass spectra recorded in the SARAMIS software (AnagnosTec, Zossen, Germany). The database was validated with 355 C. difficile strains belonging to 29 different PCR ribotypes collected prospectively from all submitted feces samples in 2009. The most frequent PCR ribotypes were type 001 (70%), 027 (4.8%) and 078/126 (4.7%). All three types were recognized by MALDI-TOF MS. We conclude that an extended MALDI-TOF system was capable to recognize specific markers for ribotypes 001, 027 and 078/126 allowing an effective identification of these strains.
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