ArticleArchives of virology2011
VereFlu™: an integrated multiplex RT-PCR and microarray assay for rapid detection and identification of human influenza A and B viruses using lab-on-chip technology.
Article in Archives of virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Seasonal Influenza: A Narrative Review of Epidemiology, Clinical Features, and Preventive Strategies.Cureus · 2025Review
- Microfluidic-based virus detection methods for respiratory diseases.Emergent materials · 2021Review
- LAMP-on-a-chip: Revising microfluidic platforms for loop-mediated DNA amplification.Trends in analytical chemistry : TRAC · 2019Review
- Diagnostic tools for tackling febrile illness and enhancing patient management.Microelectronic engineering · 2018Article
- Influenza.Nature reviews. Disease primers · 2018Review
- Genotyping of KRAS Mutational Status by the In-Check Lab-on-Chip Platform.Sensors (Basel, Switzerland) · 2018Article
- Rapid Molecular Detection and Differentiation of Influenza Viruses A and B.Journal of visualized experiments : JoVE · 2017Article
- A Novel Pan-BioMed research international · 2017Article
- A Narrative Review of Influenza: A Seasonal and Pandemic Disease.Iranian journal of medical sciences · 2017Review
- DNA Microarray Detection of 18 Important Human Blood Protozoan Species.PLoS neglected tropical diseases · 2016Article
- Performance of the Cobas(®) Influenza A/B Assay for Rapid Pcr-Based Detection of Influenza Compared to Prodesse ProFlu+ and Viral Culture.European journal of microbiology & immunology · 2015Article
- Lab-on-Chip-Based Platform for Fast Molecular Diagnosis of Multidrug-Resistant Tuberculosis.Journal of clinical microbiology · 2015Article
- DNA microarray for detection of gastrointestinal viruses.Journal of clinical microbiology · 2015Article
- Development and clinical testing of a simple, low-density gel element array for influenza identification, subtyping, and H275Y detection.Journal of virological methods · 2014Article
- Evaluation of Alere i Influenza A&B for rapid detection of influenza viruses A and B.Journal of clinical microbiology · 2014Article
- Comparative evaluation of effectiveness of IAVchip DNA microarray in influenza A diagnosis.TheScientificWorldJournal · 2014Article
- An integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens.PLoS neglected tropical diseases · 2014Article
- Profiling in situ microbial community structure with an amplification microarray.Applied and environmental microbiology · 2013Article
- Integrated Amplification Microarrays for Infectious Disease Diagnostics.Microarrays (Basel, Switzerland) · 2012Review
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11 authors.
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Abstract
Threatening sporadic outbreaks of avian influenza and the H1N1 pandemic of 2009 highlight the need for rapid and accurate detection and typing of influenza viruses. In this paper, we describe the validation of the VereFlu™ Lab-on-Chip Influenza Assay, which is based on the integration of two technologies: multiplex reverse transcription (RT)-PCR followed by microarray amplicon detection. This assay simultaneously detects five influenza virus subtypes, including the 2009 pandemic influenza A (H1N1), seasonal H1N1, H3N2, H5N1 and influenza B virus. The VereFlu™ assay was clinically validated in Singapore and compared against reference methods of real-time PCR, virus detection by immunofluorescence of cell cultures and sequencing. A sensitivity and specificity of 96.8% and 92.8%, respectively, was demonstrated for pandemic H1N1; 95.7% and 100%, respectively, for seasonal H1N1; 91.2% and 97.6%, respectively, for seasonal H3N2; 95.2% and 100%, respectively, for influenza B. Additional evaluations carried out at the World Health Organization (WHO) Collaborating Centre, Melbourne, Australia, confirmed that the test was able to reliably detect H5N1. This portable, fast time-to-answer (3 hours) device is particularly suited for diagnostic applications of detection, differentiation and identification of human influenza virus subtypes.
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