ArticleProceedings of the National Academy of Sciences of the United States of America2011
Single-molecule analysis reveals the molecular bearing mechanism of DNA strand exchange by a serine recombinase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 67 citations in OpenAlex.
- Geometry of braided DNA dictates supercoiling partitioning.Nature communications · 2026Article
- Direct observation of subunit rotation during DNA strand exchange by serine recombinases.Nature communications · 2024Article
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- Efficient targeted transgenesis of large donor DNA into multiple mouse genetic backgrounds using bacteriophage Bxb1 integrase.Scientific reports · 2022Article
- Article
- A bipartite thermodynamic-kinetic contribution by an activating mutation to RDF-independent excision by a phage serine integrase.Nucleic acids research · 2020Article
- Snapshots of a molecular swivel in action.Nucleic acids research · 2018Article
- Single-Molecule Tethered Particle Motion: Stepwise Analyses of Site-Specific DNA Recombination.Micromachines · 2018Review
- Defect-facilitated buckling in supercoiled double-helix DNA.Physical review. E · 2018Article
- Single-Molecule Magnetic Tweezer Analysis of Topoisomerases.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Nucleation of Multiple Buckled Structures in Intertwined DNA Double Helices.Physical review letters · 2017Article
- Oligomerization and ATP stimulate condensin-mediated DNA compaction.Scientific reports · 2017Article
- Control of Recombination Directionality by the Listeria Phage A118 Protein Gp44 and the Coiled-Coil Motif of Its Serine Integrase.Journal of bacteriology · 2017Article
- Torque and buckling in stretched intertwined double-helix DNAs.Physical review. E · 2017Article
- Single-molecule analysis of ϕC31 integrase-mediated site-specific recombination by tethered particle motion.Nucleic acids research · 2016Article
- Crossover-site sequence and DNA torsional stress control strand interchanges by the Bxb1 site-specific serine recombinase.Nucleic acids research · 2016Article
- The mechanism of ϕC31 integrase directionality: experimental analysis and computational modelling.Nucleic acids research · 2016Article
- Controlled rotation mechanism of DNA strand exchange by the Hin serine recombinase.Scientific reports · 2016Article
- DNA-Segment-Facilitated Dissociation of Fis and NHP6A from DNA Detected via Single-Molecule Mechanical Response.Journal of molecular biology · 2015Article
- Controlling tetramer formation, subunit rotation and DNA ligation during Hin-catalyzed DNA inversion.Nucleic acids research · 2015Article
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6 authors at 3 institutions in 1 country.
Funding
Abstract
Structural and topological data suggest that serine site-specific DNA recombinases exchange duplex DNAs by rigid-body relative rotation of the two halves of the synapse, mediated by a flat protein-protein interaction surface. We present evidence for this rotational motion for a simple serine recombinase, the Bxb1 phage integrase, from a single-DNA-based supercoil-release assay that allows us to follow crossover site cleavage, rotation, religation, and product release in real time. We have also used a two-DNA braiding-relaxation experiment to observe the effect of synapse rotation in reactions on two long molecules. Relaxation and unbraiding are rapid (averaging 54 and 70 turns/s, respectively) and complete, with no discernible pauses. Nevertheless, the molecular friction associated with rotation is larger than that of type-I topoisomerases in a similar assay. Surprisingly we find that the synapse can stay rotationally "open" for many minutes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.