ArticleJournal of immunological methods2011
Isolation of viable antigen-specific CD8+ T cells based on membrane-bound tumor necrosis factor (TNF)-α expression.
Article in Journal of immunological methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- T cells responding to Trypanosoma cruzi detected by membrane TNF-α and CD154 in chagasic patients.Immunity, inflammation and disease · 2018Trial
- The challenge and promise of studying human antigen-specific T cells.Nature reviews. Immunology · 2026Review
- Optimized procurement of cancer-reactive T-cell receptors from clinical samples.Immunotherapy advances · 2026Article
- A probiotic bacterium modulates antitumour γδ T-cell responses in lung cancer.Frontiers in immunology · 2026Article
- T cell antigenic recognition sequencing (TAR-seq) enables precise characterization of vaccine-elicited cellular immunity ex vivo andFrontiers in immunology · 2026Article
- Identification of antigen-specific functional CD8iScience · 2025Article
- The prospect of universal coronavirus immunity: a characterization of reciprocal and non-reciprocal T cell responses against SARS-CoV2 and common human coronaviruses.bioRxiv : the preprint server for biology · 2023Article
- The prospect of universal coronavirus immunity: characterization of reciprocal and non-reciprocal T cell responses against SARS-CoV2 and common human coronaviruses.Frontiers in immunology · 2023Article
- Identification and Characterization of Antigen-Specific CD8Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- Vaccine-induced ICOSCell reports. Medicine · 2021Article
- Genome-wide CRISPR-Cas9 screening reveals ubiquitous T cell cancer targeting via the monomorphic MHC class I-related protein MR1.Nature immunology · 2020Article
- Preliminary analysis of single-nucleotide polymorphisms in IL-10, IL-4, and IL-4Rα genes and profile of circulating cytokines in patients with gastric Cancer.BMC gastroenterology · 2018Article
- Peptide-MHC Class I Tetramers Can Fail To Detect Relevant Functional T Cell Clonotypes and Underestimate Antigen-Reactive T Cell Populations.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Optimized Peptide-MHC Multimer Protocols for Detection and Isolation of Autoimmune T-Cells.Frontiers in immunology · 2018Article
- T cell responses to human platelet antigen-1a involve a unique form of indirect allorecognition.JCI insight · 2016Article
- High expression of CD26 accurately identifies human bacteria-reactive MR1-restricted MAIT cells.Immunology · 2015Article
- Exhaustion of bacteria-specific CD4 T cells and microbial translocation in common variable immunodeficiency disorders.The Journal of experimental medicine · 2014Article
- MR1-restricted MAIT cells display ligand discrimination and pathogen selectivity through distinct T cell receptor usage.The Journal of experimental medicine · 2014Article
- Article
- High frequency of herpesvirus-specific clonotypes in the human T cell repertoire can remain stable over decades with minimal turnover.Journal of virology · 2013Article
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Authors and funding
8 authors.
Funding
Abstract
Current technology to isolate viable cytokine-producing antigen-specific primary human T cells is limited to bi-specific antibody capture systems, which suffer from limited sensitivity and high background. Here, we describe a novel procedure for isolating antigen-specific human T cells based on their ability to produce tumor necrosis factor (TNF)-α. Unlike many cytokines, TNF-α is initially produced in a biologically active membrane-bound form that is subsequently cleaved by TNF-α converting enzyme (TACE) to release the soluble form of TNF-α. By preventing this cleavage event, we show that TNF-α can be 'trapped' on the surface of the T cells from which it originates and directly labeled for viable isolation of these antigen-specific T cells. Together with other existing sorting procedures to isolate activated T cells, this new technique should permit the direct isolation of multi-functional T lymphocytes for further protein and gene expression analyses, as well as a detailed functional assessment of the potential role that TNF-α producing T cells play in the adaptive immune system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.