ArticleMalaria journal2011
Comparison of the in vitro invasive capabilities of Plasmodium falciparum schizonts isolated by Percoll gradient or using magnetic based separation.
Article in Malaria journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Comparative evaluation of synchronization methods for Plasmodium falciparum: Efficiency, practicality and experimental applicability.Parasites, hosts and diseases · 2026Article
- Separation of intrinsically magnetic cells using magnetic filters.Separation and purification technology · 2025Article
- Purification and production of Plasmodium falciparum zygotes from in vitro culture using magnetic column and Percoll density gradient.Malaria journal · 2020Article
- Infection of mosquitoes from in vitro cultivated Plasmodium knowlesi H strain.International journal for parasitology · 2018Article
- Comparison of two methods for transformation of Plasmodium knowlesi: Direct schizont electroporation and spontaneous plasmid uptake from plasmid-loaded red blood cells.Molecular and biochemical parasitology · 2017Article
- Validation of a chloroquine-induced cell death mechanism for clinical use against malaria.Cell death & disease · 2014Article
- Malarial hemozoin: from target to tool.Biochimica et biophysica acta · 2014Review
- Refrigeration provides a simple means to synchronize in vitro cultures of Plasmodium falciparum.Experimental parasitology · 2014Article
- Gellan sulfate inhibits Plasmodium falciparum growth and invasion of red blood cells in vitro.Scientific reports · 2014Article
- Magnetic isolation of Plasmodium falciparum schizonts iRBCs to generate a high parasitaemia and synchronized in vitro culture.Malaria journal · 2014Article
- Deep profiling of the novel intermediate-size noncoding RNAs in intraerythrocytic Plasmodium falciparum.PloS one · 2014Article
- Separation of Plasmodium falciparum late stage-infected erythrocytes by magnetic means.Journal of visualized experiments : JoVE · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPercoll gradient centrifugation is often used for synchronization, enrichment, or isolation of a particular stage of Plasmodium falciparum. However, Percoll, a hyperosmotic agent, may have harmful effects on the parasites. Magnetic bead column (MBC) separation has been used as an alternative. This is a report of a head-to-head comparison of the in vitro invasive capabilities of parasites isolated by either of the two methods.
methodsThe P. falciparum laboratory strain isolate 7G8 was grown in vitro using standard procedures and synchronized using 5% sorbitol. On separate days when the schizont parasitaemia was >1%, the culture was split and half was processed by Percoll gradient centrifugation and the other half by magnetic bead column separation. Both processed parasites were placed back in culture and allowed to invade new uninfected erythrocytes.
resultsIn 10 paired assays, the mean efficiency of invasion of 7G8 parasites treated by Percoll gradient centrifugation was 35.8% that of those treated by magnetic bead column separation (95% CI, p = 0.00067) A paired t test with two tails was used for these comparisons.
conclusionsIn this comparison, magnetic bead column separation of 7G8 schizonts resulted in higher viability and efficiency of invasion than utilizing Percoll gradient centrifugation.
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