ArticleThe Journal of biological chemistry2011
Drosophila melanogaster myosin-18 represents a highly divergent motor with actin tethering properties.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Mammalian START-like phosphatidylinositol transfer proteins - Physiological perspectives and roles in cancer biology.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2024Review
- Multifaceted Function of Myosin-18, an Unconventional Class of the Myosin Superfamily.Frontiers in cell and developmental biology · 2021Review
- Myosin 18Aα targets the guanine nucleotide exchange factor β-Pix to the dendritic spines of cerebellar Purkinje neurons and promotes spine maturation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Article
- A novel isoform of myosin 18A (Myo18Aγ) is an essential sarcomeric protein in mouse heart.The Journal of biological chemistry · 2019Article
- GOLPH3: a Golgi phosphatidylinositol(4)phosphate effector that directs vesicle trafficking and drives cancer.Journal of lipid research · 2019Review
- MYO18A: An unusual myosin.Advances in biological regulation · 2018Review
- Re-evaluating the roles of myosin 18Aα and F-actin in determining Golgi morphology.Cytoskeleton (Hoboken, N.J.) · 2017Article
- The genetic basis for variation in resistance to infection in the Drosophila melanogaster genetic reference panel.PLoS pathogens · 2017Article
- Kinetic Adaptations of Myosins for Their Diverse Cellular Functions.Traffic (Copenhagen, Denmark) · 2016Review
- Kinetic characterization of the sole nonmuscle myosin-2 from the model organism Drosophila melanogaster.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015Article
- Myosin 18A coassembles with nonmuscle myosin 2 to form mixed bipolar filaments.Current biology : CB · 2015Article
- SP-R210 (Myo18A) Isoforms as Intrinsic Modulators of Macrophage Priming and Activation.PloS one · 2015Article
- Non-muscle myosins in tumor progression, cancer cell invasion, and metastasis.Cytoskeleton (Hoboken, N.J.) · 2014Review
- Functional characterization of human myosin-18A and its interaction with F-actin and GOLPH3.The Journal of biological chemistry · 2013Article
- Mammalian myosin-18A, a highly divergent myosin.The Journal of biological chemistry · 2013Article
- Kinetic characterization of nonmuscle myosin IIb at the single molecule level.The Journal of biological chemistry · 2013Article
- Splice variant-specific cellular function of the formin INF2 in maintenance of Golgi architecture.Molecular biology of the cell · 2011Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The gene encoding Drosophila myosin-18 is complex and can potentially yield six alternatively spliced mRNAs. One of the major features of this myosin is an N-terminal PDZ domain that is included in some of the predicted alternatively spliced products. To explore the biochemical properties of this protein, we engineered two minimal motor domain (MMD)-like constructs, one that contains the N-terminal PDZ (myosin-18 M-PDZ) domain and one that does not (myosin-18 M-ΔPDZ). These two constructs were expressed in the baculovirus/Sf9 system. The results suggest that Drosophila myosin-18 is highly divergent from most other myosins in the superfamily. Neither of the MMD constructs had an actin-activated MgATPase activity, nor did they even bind ATP. Both myosin-18 M-PDZ and M-ΔPDZ proteins bound to actin with K(d) values of 2.61 and 1.04 μM, respectively, but only about 50-75% of the protein bound to actin even at high actin concentrations. Unbound proteins from these actin binding assays reiterated the 60% saturation maximum, suggesting an equilibrium between actin-binding and non-actin-binding conformations of Drosophila myosin-18 in vitro. Neither the binding affinity nor the substoichiometric binding was significantly affected by ATP. Optical trapping of single molecules in three-bead assays showed short lived interactions of the myosin-18 motors with actin filaments. Combined, these data suggest that this highly divergent motor may function as an actin tethering protein.
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