ArticleApplied and environmental microbiology2011
Rapid detection of viable salmonellae in produce by coupling propidium monoazide with loop-mediated isothermal amplification.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 173 citations in OpenAlex.
- Development and validation of a rapidFrontiers in microbiology · 2026Article
- Comparative evaluation of 14Frontiers in microbiology · 2026Article
- Loop-Mediated Isothermal Amplification Assay for Visual Detection ofFoods (Basel, Switzerland) · 2025Article
- Article
- Electrospun Nanofiber-Based Biosensors for Foodborne Bacteria Detection.Molecules (Basel, Switzerland) · 2024Review
- Research progress of loop-mediated isothermal amplification in the detection of Salmonella for food safety applications.Discover nano · 2024Review
- Comparative assessment of two in-house-built isothermal assays for visual detection of African swine fever virus.Journal of biosciences · 2024Article
- DNA from non-viable bacteria biases diversity estimates in the corals Acropora loripes and Pocillopora acuta.Environmental microbiome · 2023Article
- Advances in detection methods for viable Salmonella spp.: current applications and challenges.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Review
- Current and Future Advances in the Detection and Surveillance of Biosecurity-Relevant Equine Bacterial Diseases Using Loop-Mediated Isothermal Amplification (LAMP).Animals : an open access journal from MDPI · 2023Review
- Shift of human pathogen community composition and their potential human health risk after supply suspension in tap water.Scientific reports · 2023Article
- Label-Free Sequence-Specific Visualization of LAMP AmplifiedBiosensors · 2023Article
- A Real-Time PCR Approach for Rapid Detection of ViableMicroorganisms · 2023Article
- Toward the Adoption of Loop-Mediated Isothermal Amplification forFoodborne pathogens and disease · 2022Article
- Simultaneous detection of viableFood science & nutrition · 2022Article
- Changes in physiological states of Salmonella Typhimurium measured by qPCR with PMA and DyeTox13 Green Azide after pasteurization and UV treatment.Applied microbiology and biotechnology · 2022Article
- A new method for the rapid detection of the antibacterial and bacteriostatic activity of disinfectants based on Propidium Monoazide combined with real-time PCR.Frontiers in microbiology · 2022Article
- Monitoring of methylene blue monomers and dimers to control the bacterialogical water quality including application to photocatalysis.Environmental science and pollution research international · 2021Article
- Simultaneous detection of Salmonella spp., Pseudomonas aeruginosa, Bacillus cereus, and Escherichia coli O157:H7 in environmental water using PMA combined with mPCR.Journal of microbiology (Seoul, Korea) · 2020Article
- Optimization of isothermal amplification method for Mycobacteriumtuberculosisdetection and visualization method for fieldworkTurkish journal of medical sciences · 2020Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent outbreaks linked to Salmonella-contaminated produce heightened the need to develop simple, rapid, and accurate detection methods, particularly those capable of determining cell viability. In this study, we examined a novel strategy for the rapid detection and quantification of viable salmonellae in produce by coupling a simple propidium monoazide sample treatment with loop-mediated isothermal amplification (PMA-LAMP). We first designed and optimized a LAMP assay targeting Salmonella. Second, the performance of PMA-LAMP for detecting and quantifying viable salmonellae was determined. Finally, the assay was evaluated in experimentally contaminated produce items (cantaloupe, spinach, and tomato). Under the optimized condition, PMA-LAMP consistently gave negative results for heat-killed Salmonella cells with concentrations up to 10(8) CFU/ml (or CFU/g in produce). The detection limits of PMA-LAMP were 3.4 to 34 viable Salmonella cells in pure culture and 6.1 × 10(3) to 6.1 × 10(4) CFU/g in spiked produce samples. In comparison, PMA-PCR was up to 100-fold less sensitive. The correlation between LAMP time threshold (T(T)) values and viable Salmonella cell numbers was high (R(2) = 0.949 to 0.993), with a quantification range (10(2) to 10(5) CFU/reaction in pure culture and 10(4) to 10(7) CFU/g in produce) comparable to that of PMA in combination with quantitative real-time PCR (PMA-qPCR). The complete PMA-LAMP assay took about 3 h to complete when testing produce samples. In conclusion, this rapid, accurate, and simple method to detect and quantify viable Salmonella cells in produce may present a useful tool for the produce industry to better control potential microbial hazards in produce.
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