ArticleJournal of bacteriology2011
Molecular and biochemical characterization of the 5-nitroanthranilic acid degradation pathway in Bradyrhizobium sp. strain JS329.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Adaptations of the GenusCurrent issues in molecular biology · 2025Review
- Article
- Crystal structure of the monocupin ring-cleaving dioxygenase 5-nitrosalicylate 1,2-dioxygenase from Bradyrhizobium sp.Acta crystallographica. Section D, Structural biology · 2023Article
- A Biomimetic System for Studying Salicylate Dioxygenase.ACS symposium series. American Chemical Society · 2019Article
- Response of Gut Microbiota to Metabolite Changes Induced by Endurance Exercise.Frontiers in microbiology · 2018Article
- Petroleum Contamination and Plant Identity Influence Soil and Root Microbial Communities While AMF Spores Retrieved from the Same Plants Possess Markedly Different Communities.Frontiers in plant science · 2017Article
- Enzymatic hydrolysis by transition-metal-dependent nucleophilic aromatic substitution.Nature chemical biology · 2016Article
- Bacterial degradation of monocyclic aromatic amines.Frontiers in microbiology · 2015Review
- Operational performance, biomass and microbial community structure: impacts of backwashing on drinking water biofilter.Environmental science and pollution research international · 2015Article
- Structure and function of atypically coordinated enzymatic mononuclear non-heme-Fe(II) centers.Coordination chemistry reviews · 2013Review
- Aerobic degradation of N-methyl-4-nitroaniline (MNA) by Pseudomonas sp. strain FK357 isolated from soil.PloS one · 2013Article
- Ring-cleaving dioxygenases with a cupin fold.Applied and environmental microbiology · 2012Review
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biodegradation pathways of synthetic nitroaromatic compounds and anilines are well documented, but little is known about those of nitroanilines. We previously reported that the initial step in 5-nitroanthranilic acid (5NAA) degradation by Bradyrhizobium sp. strain JS329 is a hydrolytic deamination to form 5-nitrosalicylic acid (5NSA), followed by ring fission catalyzed by 5NSA dioxygenase. The mechanism of release of the nitro group was unknown. In this study, we subcloned, sequenced, and expressed the genes encoding 5NAA deaminase (5NAA aminohydrolase, NaaA), 5NSA dioxygenase (NaaB) and lactonase (NaaC), the key genes responsible for 5NAA degradation. Sequence analysis and enzyme characterization revealed that NaaA is a hydrolytic metalloenzyme with a narrow substrate range. The nitro group is spontaneously eliminated as nitrite concomitant with the formation of a lactone from the ring fission product of 5NSA dioxygenation. The elimination of the nitro group during lactone formation is a previously unreported mechanism for denitration of nitro aliphatic compounds.
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