ReviewCurrent opinion in chemical biology2011
Protein stability by number: high-throughput and statistical approaches to one of protein science's most difficult problems.
Review in Current opinion in chemical biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Fluorescence-Based Protein Stability Monitoring-A Review.International journal of molecular sciences · 2024Review
- Hidden information on protein function in censuses of proteome foldedness.Nature communications · 2022Article
- Therapeutic enzyme engineering using a generative neural network.Scientific reports · 2022Article
- Is Protein Folding a Thermodynamically Unfavorable, Active, Energy-Dependent Process?International journal of molecular sciences · 2022Review
- Slowest-first protein translation scheme: Structural asymmetry and co-translational folding.Biophysical journal · 2021Article
- SAAFEC-SEQ: A Sequence-Based Method for Predicting the Effect of Single Point Mutations on Protein Thermodynamic Stability.International journal of molecular sciences · 2021Article
- A Thermodynamic Model for Interpreting Tryptophan Excitation-Energy-Dependent Fluorescence Spectra Provides Insight Into Protein Conformational Sampling and Stability.Frontiers in molecular biosciences · 2021Article
- Biophysical Inference of Epistasis and the Effects of Mutations on Protein Stability and Function.Molecular biology and evolution · 2018Article
- Modeling protein folding in vivo.Biology direct · 2018Article
- Protein Evolution is Potentially Governed by Protein Stability: Directed Evolution of an Esterase from the Hyperthermophilic Archaeon Sulfolobus tokodaii.Journal of molecular evolution · 2018Article
- SDM: a server for predicting effects of mutations on protein stability.Nucleic acids research · 2017Article
- Consensus protein design.Protein engineering, design & selection : PEDS · 2016Review
- High-throughput identification of protein mutant stability computed from a double mutant fitness landscape.Protein science : a publication of the Protein Society · 2016Article
- Protein stability: computation, sequence statistics, and new experimental methods.Current opinion in structural biology · 2015Review
- Structural and dynamic properties that govern the stability of an engineered fibronectin type III domain.Protein engineering, design & selection : PEDS · 2015Article
- Protein evolution analysis of S-hydroxynitrile lyase by complete sequence design utilizing the INTMSAlign software.Scientific reports · 2015Article
- Deep mutational scanning: a new style of protein science.Nature methods · 2014Article
- Consensus design of a NOD receptor leucine rich repeat domain with binding affinity for a muramyl dipeptide, a bacterial cell wall fragment.Protein science : a publication of the Protein Society · 2014Article
- Ligands for glaucoma-associated myocilin discovered by a generic binding assay.ACS chemical biology · 2014Article
- Optically induced thermal gradients for protein characterization in nanolitre-scale samples in microfluidic devices.Scientific reports · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Most proteins are only barely stable, which impedes research, complicates therapeutic applications, and makes proteins susceptible to pathologically destabilizing mutations. Our ability to predict the thermodynamic consequences of even single point mutations is still surprisingly limited, and established methods of measuring stability are slow. Recent advances are bringing protein stability studies into the high-throughput realm. Some methods are based on inferential read-outs such as activity, proteolytic resistance or split-protein fragment reassembly. Other methods use miniaturization of direct measurements, such as intrinsic fluorescence, H/D exchange, cysteine reactivity, aggregation and hydrophobic dye binding (DSF). Protein engineering based on statistical analysis (consensus and correlated occurrences of amino acids) is promising, but much work remains to understand and implement these methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.