ArticleJournal of neuroscience methods2011
Random insertion of split-cans of the fluorescent protein venus into Shaker channels yields voltage sensitive probes with improved membrane localization in mammalian cells.
Article in Journal of neuroscience methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
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- AGS3-based optogenetic GDI induces GPCR-independent Gβγ signaling and macrophage migration.bioRxiv : the preprint server for biology · 2024Article
- Advancements in the Quest to Map, Monitor, and Manipulate Neural Circuitry.Frontiers in neural circuits · 2022Review
- Proteome plasticity in response to persistent environmental change.Molecular cell · 2021Article
- Protein Topology Prediction Algorithms Systematically Investigated in the Yeast Saccharomyces cerevisiae.BioEssays : news and reviews in molecular, cellular and developmental biology · 2019Article
- Ataxia in Patients With Bi-AllelicFrontiers in genetics · 2019Article
- Genome-wide SWAp-Tag yeast libraries for proteome exploration.Nature methods · 2018Article
- Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight.ACS chemical neuroscience · 2017Article
- Design and development of genetically encoded fluorescent sensors to monitor intracellular chemical and physical parameters.Biophysical reviews · 2016Review
- Red fluorescent proteins (RFPs) and RFP-based biosensors for neuronal imaging applications.Neurophotonics · 2015Article
- Personal recollections: regarding the pioneer days of optical recording of membrane potential using voltage-sensitive dyes.Neurophotonics · 2015Article
- Mechanistic studies of the genetically encoded fluorescent protein voltage probe ArcLight.PloS one · 2014Article
- Probing neuronal activities with genetically encoded optical indicators: from a historical to a forward-looking perspective.Pflugers Archiv : European journal of physiology · 2013Review
- Fluorescent protein voltage probes derived from ArcLight that respond to membrane voltage changes with fast kinetics.PloS one · 2013Article
- Article
- Genetically engineered fluorescent voltage reporters.ACS chemical neuroscience · 2012Review
- Two-photon scanning microscopy of in vivo sensory responses of cortical neurons genetically encoded with a fluorescent voltage sensor in rat.Frontiers in neural circuits · 2012Article
- A fluorescent, genetically-encoded voltage probe capable of resolving action potentials.PloS one · 2012Article
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
FlaSh-YFP, a fluorescent protein (FP) voltage sensor that is a fusion of the Shaker potassium channel with yellow fluorescent protein (YFP), is primarily expressed in the endoplasmic reticulum (ER) of mammalian cells, possibly due to misfolded monomers. In an effort to improve plasma membrane expression, the FP was split into two non-fluorescent halves. Each half was randomly inserted into Shaker monomers via a transposon reaction. Shaker subunits containing the 5' half were co-expressed with Shaker subunits containing the 3' half. Tetramerization of Shaker subunits is required for re-conjugation of the FP. The misfolded monomers trapped in ER are unlikely to tetramerize and reconstitute the beta-can structure, and thus intracellular fluorescence might be reduced. This split-can transposon approach yielded 56 fluorescent probes, 30 (54%) of which were expressed at the plasma membrane and were capable of optically reporting changes in membrane potential. The largest signal from these novel FP-sensors was a -1.4% in ΔF/F for a 100 mV depolarization, with on time constants of about 15 ms and off time constants of about 200 ms. This split-can transposon approach has the potential to improve other multimeric probes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.