ArticleJournal of neuroscience methods2011
A simple method for measuring organotypic tissue slice culture thickness.
Article in Journal of neuroscience methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Potential of ex vivo organotypic slice cultures in neuro-oncology.Neuro-oncology · 2025Review
- A microfluidic chip for sustained oxygen gradient formation in the intestineLab on a chip · 2024Article
- Organotypic whole hemisphere brain slice models to study the effects of donor age and oxygen-glucose-deprivation on the extracellular properties of cortical and striatal tissue.Journal of biological engineering · 2022Article
- Alternative Brain Slice-on-a-Chip for Organotypic Culture and Effective Fluorescence Injection Testing.International journal of molecular sciences · 2022Article
- Long-Lived Organotypic Slice Culture Model of the Rat Basolateral Amygdala.Current protocols · 2021Article
- Evaluation of cell viability and metabolic activity of a 3D cultured human epidermal model using a dynamic autoradiographic technique with a PET radiopharmaceutical.Scientific reports · 2019Article
- Tight coupling of astrocyte energy metabolism to synaptic activity revealed by genetically encoded FRET nanosensors in hippocampal tissue.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2019Article
- Increasing cellular lifespan with a flow system in organotypic culture of the Laterodorsal Tegmentum (LDT).Scientific reports · 2019Article
- Electrokinetic infusions into hydrogels and brain tissue: Control of direction and magnitude of solute delivery.Journal of neuroscience methods · 2019Article
- Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells.Journal of visualized experiments : JoVE · 2017Article
- Organotypic brain slice cultures: A review.Neuroscience · 2015Review
- Iontophoresis from a micropipet into a porous medium depends on the ζ-potential of the medium.Analytical chemistry · 2012Article
- A 6-Step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model.Basic and clinical neuroscienceArticle
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Authors and funding
4 authors.
Funding
Abstract
This paper presents a simple method to measure tissue slice thicknesses using an ohmmeter. The circuit described here is composed of a metal probe, an ohmmeter, a counter electrode, culture medium or physiological buffer, and tissue slice. The probe and the electrode are on opposite interfaces of an organotypic hippocampal slice culture. The circuit closes when the metal probe makes contact with the surface of the tissue slice. The probe position is recorded and compared to its position when it makes contact with the insert membrane on which the tissue grows, thus yielding a thickness measurement. The method does not reduce the viability of slice cultures. Thicknesses of the slice cultures were measured under a number of culturing protocols. An initial drop in thickness occurred between 0 and 4 days in culture. Thicknesses are rather constant thereafter. The type of culture medium and the initial thickness of the tissue explant influence the thickness. Slice thicknesses were compared to a known technique by using optical measurements of slice cross-sections to obtain thicknesses. In contrast to this known technique, the proposed method does not sacrifice the slice culture for measurement purposes. The proposed measurement technique described is straightforward and rapid, about 1 min per culture.
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