ArticleFertility and sterility2011
Gonadotropin-releasing hormone agonist increases expression of osmotic response genes in leiomyoma cells.
Article in Fertility and sterility, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Ulipristal Acetate and Extracellular Matrix Production in Human Leiomyomas In Vivo: A Laboratory Analysis of a Randomized Placebo Controlled Trial.Reproductive sciences (Thousand Oaks, Calif.) · 2018Trial
- NFAT5: a stress-related transcription factor with multiple functions in health and disease.Cell stress · 2025Review
- Review
- Recurrent Amplification of the Osmotic Stress Transcription FactorJournal of the Endocrine Society · 2020Article
- Ulipristal Acetate Mediates Decreased Proteoglycan Expression Through Regulation of Nuclear Factor of Activated T-Cells (NFAT5).Reproductive sciences (Thousand Oaks, Calif.) · 2019Article
- Mifepristone inhibits IGF-1 signaling pathway in the treatment of uterine leiomyomas.Drug design, development and therapy · 2019Article
- Article
- The use of mifepristone in abortion associated with an increased risk of uterine leiomyomas.Medicine · 2017Observational
- Autocrine MCP-1/CCR2 signaling stimulates proliferation and migration of renal carcinoma cells.Oncology letters · 2016Article
- Dynamic reciprocity between cells and their microenvironment in reproduction.Biology of reproduction · 2015Review
- Mechanical signaling in reproductive tissues: mechanisms and importance.Reproductive sciences (Thousand Oaks, Calif.) · 2014Review
- Expression of a mitochondrial progesterone receptor (PR-M) in leiomyomata and association with increased mitochondrial membrane potential.The Journal of clinical endocrinology and metabolism · 2014Article
- NFAT5-mediated expression of S100A4 contributes to proliferation and migration of renal carcinoma cells.Frontiers in physiology · 2014Article
- The extracellular matrix contributes to mechanotransduction in uterine fibroids.Obstetrics and gynecology international · 2014Review
- Characterization of tissue biomechanics and mechanical signaling in uterine leiomyoma.Matrix biology : journal of the International Society for Matrix Biology · 2012Article
- Treatment for Uterine Fibroids: Searching for Effective Drug Therapies.Drug discovery today. Therapeutic strategies · 2012Article
- Proceedings from the Third National Institutes of Health International Congress on Advances in Uterine Leiomyoma Research: comprehensive review, conference summary and future recommendations.Human reproduction updateArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveTo characterize hyperosmolarity-responsive genes in leiomyoma cells and determine whether gonadotropin-releasing hormone (GnRH) agonist treatment altered their expression.
designLaboratory study.
settingUniversity hospital. PATIENT(S): None. INTERVENTION(S): Cell culture under hypertonic conditions and with GnRH agonist treatment, RNA isolation, and real-time reverse-transcriptase polymerase chain reaction (RT-PCR). MAIN OUTCOME MEASURE(S): Expression of nuclear factor of activated T cells 5 (NFAT5), aldose reductase (AR), and sodium myo-inositol transporter 1 (SMIT) messenger RNA (mRNA) in immortalized leiomyoma and patient-matched myometrial cells. RESULT(S): Leiomyoma cells had increased basal expression of NFAT5 mRNA (1.7±0.08-fold) compared with myometrial cells. The NFAT5 increased further in leiomyoma cells cultured under hyperosmolar conditions (3.0±0.46-fold at 50 mM NaCl and 3.3±0.48-fold at 100 mM NaCl). The NFAT5-regulated mRNA transcripts for AR and SMIT were increased in untreated leiomyoma cells compared with myometrial cells and further increased in leiomyoma cells exposed to osmotic stress. The NFAT5 transcripts were decreased with low-dose GnRH agonist treatment but increased with supraphysiologic doses. CONCLUSION(S): Expression of hyperosmolarity genes was increased in leiomyoma cells relative to myometrial cells. Pharmacologic concentrations of GnRH agonist decreased NFAT5 expression, suggesting that water flows out of leiomyoma cells at pharmacologic doses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.