ArticleMolecular imaging2011
ATP-binding cassette transporters modulate both coelenterazine- and D-luciferin-based bioluminescence imaging.
Article in Molecular imaging, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 17 citations in OpenAlex.
- Matrix Effects on the Quantification of NanoLuc Bioluminescence in Ex Vivo Organ Homogenates.Sensors (Basel, Switzerland) · 2026Article
- Identification of NanoLuciferase Substrates Transported by Human ABCB1 and ABCG2 and Their Zebrafish Homologs at the Blood-Brain Barrier.Molecular pharmacology · 2024Article
- Identification of NanoLuciferase Substrates Transported by Human ABCB1 and ABCG2 and their Zebrafish Homologs at the Blood-Brain Barrier.bioRxiv : the preprint server for biology · 2023Article
- A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice.Nature communications · 2022Article
- Berberine Reverses Breast Cancer Multidrug Resistance Based on Fluorescence PharmacokineticsACS omega · 2021Article
- Interaction of Styrylpyridinium Compound with PathogenicMicroorganisms · 2020Article
- Study on mechanism of elemene reversing tumor multidrug resistance based on luminescence pharmacokinetics in tumor cellsRSC advances · 2020Article
- Enhancement of PSMA-Directed CAR Adoptive Immunotherapy by PD-1/PD-L1 Blockade.Molecular therapy oncolytics · 2017Article
- Detection of Lymph Node Metastases with SERRS Nanoparticles.Molecular imaging and biology · 2016Article
- Combining fluorescence and bioluminescence microscopy.Microscopy research and technique · 2015Article
- Molecular Imaging of Membrane Transporters' Activity in Cancer: a Picture is Worth a Thousand Tubes.The AAPS journal · 2015Review
- Pre- and postmortem imaging of transplanted cells.International journal of nanomedicine · 2015Review
- Oatp1 enhances bioluminescence by acting as a plasma membrane transporter for D-luciferin.Molecular imaging and biology · 2014Article
- Dual systemic tumor targeting with ligand-directed phage and Grp78 promoter induces tumor regression.Molecular cancer therapeutics · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Bioluminescence imaging (BLI) of luciferase reporters provides a cost-effective and sensitive means to image biological processes. However, transport of luciferase substrates across the cell membrane does affect BLI readout intensity from intact living cells. To investigate the effect of ATP-binding cassette (ABC) transporters on BLI readout, we generated click beetle (cLuc), firefly (fLuc), Renilla (rLuc), and Gaussia (gLuc) luciferase HEK-293 reporter cells that overexpressed different ABC transporters (ABCB1, ABCC1, and ABCG2). In vitro studies showed a significant BLI intensity decrease in intact cells compared to cell lysates, when ABCG2 was overexpressed in HEK-293/cLuc, fLuc, and rLuc cells. Selective ABC transporter inhibitors were also applied. Inhibition of ABCG2 activity increased the BLI intensity more than two-fold in HEK-293/cLuc, fLuc, and rLuc cells; inhibition of ABCB1 elevated the BLI intensity two-fold only in HEK-293/rLuc cells. BLI of xenografts derived from HEK-293/ABC transporter/luciferase reporter cells confirmed the results of inhibitor treatment in vivo. These findings demonstrate that coelenterazine-based rLuc-BLI intensity can be modulated by ABCB1 and ABCG2. ABCG2 modulates d-luciferin-based BLI in a luciferase type-independent manner. Little ABC transporter effect on gLuc-BLI intensity is observed because a large fraction of gLuc is secreted. The expression level of ABC transporters is one key factor affecting BLI intensity, and this may be particularly important in luciferase-based applications in stem cell research.
Indexed as
Identifiers
21496450PMC4052835W2160564459What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.