ArticlePloS one2011
Ultra-fast and sensitive detection of non-typhoidal Salmonella using microwave-accelerated metal-enhanced fluorescence ("MAMEF").
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Rapid, Point-of-Care Microwave Lysis and Electrochemical Detection ofBioengineering (Basel, Switzerland) · 2024Article
- Microwaves, a potential treatment for bacteria: A review.Frontiers in microbiology · 2022Review
- Applications of Microwave Energy in Medicine.Biosensors · 2021Review
- Typhoid fever: Control & challenges in India.The Indian journal of medical research · 2019Review
- Non-typhoidal Salmonella blood stream infection in Kuwait: Clinical and microbiological characteristics.PLoS neglected tropical diseases · 2019Article
- Rapid sample preparation with Lyse-It® for Listeria monocytogenes and Vibrio cholerae.PloS one · 2018Article
- Microwave-accelerated method for ultra-rapid extraction of Neisseria gonorrhoeae DNA for downstream detection.Analytical biochemistry · 2016Article
- Detection of Typhoidal and Paratyphoidal Salmonella in Blood by Real-time Polymerase Chain Reaction.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2015Article
- Diagnostics for invasive Salmonella infections: Current challenges and future directions.Vaccine · 2015Review
- Adaptation of red blood cell lysis represents a fundamental breakthrough that improves the sensitivity of Salmonella detection in blood.Journal of applied microbiology · 2015Article
- Relationship between antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya.PLoS neglected tropical diseases · 2015Article
- 5-color multiplexed microwave-accelerated metal-enhanced fluorescence: detection and analysis of multiple DNA sequences from within one sample well within a few seconds.Journal of fluorescence · 2014Article
- Sensitive detection and serovar differentiation of typhoidal and nontyphoidal Salmonella enterica species using 16S rRNA Gene PCR coupled with high-resolution melt analysis.The Journal of molecular diagnostics : JMD · 2014Article
- Article
- Blind evaluation of the microwave-accelerated metal-enhanced fluorescence ultrarapid and sensitive Chlamydia trachomatis test by use of clinical samples.Journal of clinical microbiology · 2013Article
- Highly sensitive quantitation of human serum albumin in clinical samples for hypoproteinemia using metal-enhanced fluorescence.Journal of fluorescence · 2013Article
- Reduced lifetimes are directly correlated with excitation irradiance in metal-enhanced fluorescence (MEF).Journal of fluorescence · 2012Article
- Invasive non-typhoidal salmonella disease: an emerging and neglected tropical disease in Africa.Lancet (London, England) · 2012Review
- Invasive nontyphoidal Salmonella disease: epidemiology, pathogenesis and diagnosis.Current opinion in infectious diseases · 2011Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Certain serovars of Salmonella enterica subsp. enterica cause invasive disease (e.g., enteric fever, bacteremia, septicemia, meningitis, etc.) in humans and constitute a global public health problem. A rapid, sensitive diagnostic test is needed to allow prompt initiation of therapy in individual patients and for measuring disease burden at the population level. An innovative and promising new rapid diagnostic technique is microwave-accelerated metal-enhanced fluorescence (MAMEF). We have adapted this assay platform to detect the chromosomal oriC locus common to all Salmonella enterica subsp. enterica serovars. We have shown efficient lysis of biologically relevant concentrations of Salmonella spp. suspended in bacteriological media using microwave-induced lysis. Following lysis and DNA release, as little as 1 CFU of Salmonella in 1 ml of medium can be detected in <30 seconds. Furthermore the assay is sensitive and specific: it can detect oriC from Salmonella serovars Typhi, Paratyphi A, Paratyphi B, Paratyphi C, Typhimurium, Enteritidis and Choleraesuis but does not detect Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae or Acinetobacter baumanii. We have also performed preliminary experiments using a synthetic Salmonella oriC oligonucleotide suspended in whole human blood and observed rapid detection when the sample was diluted 1:1 with PBS. These pre-clinical data encourage progress to the next step to detect Salmonella in blood (and other ordinarily sterile, clinically relevant body fluids).
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Registered trials
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