ArticlePloS one2011
Ptenb mediates gastrulation cell movements via Cdc42/AKT1 in zebrafish.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Gene editing in monogenic autism spectrum disorder: animal models and gene therapies.Frontiers in molecular neuroscience · 2022Review
- Review
- Wnt5b integrates Fak1a to mediate gastrulation cell movements via Rac1 and Cdc42.Open biology · 2020Article
- IRE1α governs cytoskeleton remodelling and cell migration through a direct interaction with filamin A.Nature cell biology · 2018Article
- Cdc42 controls primary mesenchyme cell morphogenesis in the sea urchin embryo.Developmental biology · 2018Article
- AKT1 restricts the invasive capacity of head and neck carcinoma cells harboring a constitutively active PI3 kinase activity.BMC cancer · 2018Article
- A GAL4-inducible transgenic tool kit for the in vivo modulation of Rho GTPase activity in zebrafish.Developmental dynamics : an official publication of the American Association of Anatomists · 2016Article
- Function Over Form: Modeling Groups of Inherited Neurological Conditions in Zebrafish.Frontiers in molecular neuroscience · 2016Review
- The Rho GTPase Cdc42 regulates hair cell planar polarity and cellular patterning in the developing cochlea.Biology open · 2015Article
- Characterizing informative sequence descriptors and predicting binding affinities of heterodimeric protein complexes.BMC bioinformatics · 2015Article
- Pten regulates homeostasis and inflammation-induced migration of myelocytes in zebrafish.Journal of hematology & oncology · 2014Article
- Genome wide analysis reveals Zic3 interaction with distal regulatory elements of stage specific developmental genes in zebrafish.PLoS genetics · 2013Article
- Zebrafish rgs4 is essential for motility and axonogenesis mediated by Akt signaling.Cellular and molecular life sciences : CMLS · 2013Article
- Pten regulates collective cell migration during specification of the anterior-posterior axis of the mouse embryo.Developmental biology · 2012Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphatidylinositol 3-kinase (PI3 kinase) mediates gastrulation cell migration in zebrafish via its regulation of PIP(2)/PIP(3) balance. Although PI3 kinase counter enzyme PTEN has also been reported to be essential for gastrulation, its role in zebrafish gastrulation has been controversial due to the lack of gastrulation defects in pten-null mutants. To clarify this issue, we knocked down a pten isoform, ptenb by using anti-sense morpholino oligos (MOs) in zebrafish embryos and found that ptenb MOs inhibit convergent extension by affecting cell motility and protrusion during gastrulation. The ptenb MO-induced convergence defect could be rescued by a PI3-kinase inhibitor, LY294002 and by overexpressing dominant negative Cdc42. Overexpression of human constitutively active akt1 showed similar convergent extension defects in zebrafish embryos. We also observed a clear enhancement of actin polymerization in ptenb morphants under cofocal microscopy and in actin polymerization assay. These results suggest that Ptenb by antagonizing PI3 kinase and its downstream Akt1 and Cdc42 to regulate actin polymerization that is critical for proper cell motility and migration control during gastrulation in zebrafish.
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