ArticleBreast cancer : basic and clinical research2011
Optimized Protocol for Protein Extraction from the Breast Tissue that is Compatible with Two-Dimensional Gel Electrophoresis.
Article in Breast cancer : basic and clinical research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Humoral immunoprofiling identifies novel biomarkers and an immune suppressive autoantibody phenotype at the site of disease in pancreatic ductal adenocarcinoma.Frontiers in oncology · 2024Article
- Human adipose and skeletal muscle tissue DNA, RNA, and protein content.Journal of applied physiology (Bethesda, Md. : 1985) · 2021Article
- Plasma extracellular vesicles detected by Single Molecule array technology as a liquid biopsy for colorectal cancer.Journal of extracellular vesicles · 2020Article
- Over-expression of mammaglobin-B in canine mammary tumors.BMC veterinary research · 2018Article
- The R-Enantiomer of Ketorolac Delays Mammary Tumor Development in Mouse Mammary Tumor Virus-Polyoma Middle T Antigen (MMTV-PyMT) Mice.The American journal of pathology · 2018Article
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Authors and funding
4 authors.
Funding
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Abstract
Proteomics is a highly informative approach to analyze cancer-associated transformation in tissues. The main challenge to use a tissue for proteomics studies is the small sample size and difficulties to extract and preserve proteins. The choice of a buffer compatible with proteomics applications is also a challenge. Here we describe a protocol optimized for the most efficient extraction of proteins from the human breast tissue in a buffer compatible with two-dimensional gel electrophoresis (2D-GE). This protocol is based on mechanically assisted disintegration of tissues directly in the 2D-GE buffer. Our method is simple, robust and easy to apply in clinical practice. We demonstrate high quality of separation of proteins prepared according to the reported here protocol.
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