ArticlePloS one2011
Biochemical and structural properties of cyanases from Arabidopsis thaliana and Oryza sativa.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Structural mechanism of Escherichia coli cyanase.Acta crystallographica. Section D, Structural biology · 2023Article
- Cyanogenesis, a Plant Defence Strategy against Herbivores.International journal of molecular sciences · 2023Review
- Heterologous Expression of Cyanobacterial Cyanase Gene (Biology · 2022Article
- Hydroxynitrile lyase defends Arabidopsis against Tetranychus urticae.Plant physiology · 2022Article
- Crystal structure of a thermophilic fungal cyanase and its implications on the catalytic mechanism for bioremediation.Scientific reports · 2021Article
- Interaction of cyanate uptake by rice seedlings with nitrate assimilation: gene expression analysis.Environmental science and pollution research international · 2019Article
- Cyanase-independent utilization of cyanate as a nitrogen source in ascomycete yeasts.World journal of microbiology & biotechnology · 2018Article
- Expression of the cyanobacterial enzyme cyanase increases cyanate metabolism and cyanate tolerance in Arabidopsis.Environmental science and pollution research international · 2017Article
- Proteomic profiling of maize opaque endosperm mutants reveals selective accumulation of lysine-enriched proteins.Journal of experimental botany · 2016Article
- Serendipitous crystallization and structure determination of cyanase (CynS) from Serratia proteamaculans.Acta crystallographica. Section F, Structural biology communications · 2015Article
- 2D-DIGE-based proteome expression changes in leaves of rice seedlings exposed to low-level gamma radiation at Iitate village, Fukushima.Plant signaling & behavior · 2015Article
- Poles apart: Arctic and Antarctic Octadecabacter strains share high genome plasticity and a new type of xanthorhodopsin.PloS one · 2013Article
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5 authors.
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Abstract
Cyanate is toxic to all organisms. Cyanase converts cyanate to CO₂ and NH₃ in a bicarbonate-dependent reaction. The biophysical functions and biochemical characteristics of plant cyanases are poorly studied, although it has been investigated in a variety of proteobacteria, cyanobacteria and fungi. In this study, we characterised plant cyanases from Arabidopsis thaliana and Oryza sativa (AtCYN and OsCYN). Prokaryotic-expressed AtCYN and OsCYN both showed cyanase activity in vitro. Temperature had a similar influence on the activity of both cyanases, but pH had a differential impact on AtCYN and OsCYN activity. Homology modelling provided models of monomers of AtCYN and OsCYN, and a coimmunoprecipitation assay and gel filtration indicated that AtCYN and OsCYN formed homodecamers. The analysis of single-residue mutants of AtCYN indicated that the conserved catalytic residues also contributed to the stability of the homodecamer. KCNO treatment inhibited Arabidopsis germination and early seedling growth. Plants containing AtCYN or OsCYN exhibited resistance to KCNO stress, which demonstrated that one role of cyanases in plants is detoxification. Transcription level of AtCYN was higher in the flower than in other organs of Arabidopsis. AtCYN transcription was not significantly affected by KCNO treatment in Arabidopsis, but was induced by salt stress. This research broadens our knowledge on plant detoxification of cyanate via cyanase.
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