ArticleVirology journal2011
Establishment of persistent infection with foot-and-mouth disease virus in BHK-21 cells.
Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 51 citations in OpenAlex.
- Integrated multiomics reveals host-virus coadaptation in persistent foot-and-mouth disease virus infection.Cellular and molecular life sciences : CMLS · 2025Article
- Application ofVeterinary sciences · 2025Article
- Inactivation of an Indonesian isolate of foot-and-mouth disease virus using formaldehyde.Veterinary world · 2024Article
- Preparation of purified vaccine from local isolate of foot and mouth disease virus and its immune response in bovine calves.Saudi journal of biological sciences · 2023Article
- Single-Cell Sequencing Yields Insights in the Evolution of Foot-and-Mouth Disease Virus Persistent Infection.Frontiers in cellular and infection microbiology · 2022Article
- Vaccine matching and antigenic variability of foot-and-mouth disease virus serotypes O and A from 2018 Ethiopian isolates.International microbiology : the official journal of the Spanish Society for Microbiology · 2022Article
- A Membrane-Targeting Photosensitizer with Aggregation-Induced Emission Characteristics for Highly Efficient Photodynamic Combat of Human Coronaviruses.Small (Weinheim an der Bergstrasse, Germany) · 2021Article
- Molecular characterization of foot-and-mouth disease viruses collected from Northern and Central Ethiopia during the 2018 outbreak.Veterinary world · 2020Article
- The Carrier Conundrum; A Review of Recent Advances and Persistent Gaps Regarding the Carrier State of Foot-and-Mouth Disease Virus.Pathogens (Basel, Switzerland) · 2020Review
- Comparative Transcriptome Analysis Reveals Different Host Cell Responses to Acute and Persistent Foot-and-Mouth Disease Virus Infection.Virologica Sinica · 2020Article
- Single-Cell Analysis of Foot-and-Mouth Disease Virus.Frontiers in microbiology · 2020Review
- Isolation, Serotyping, and Molecular Detection of Bovine FMD Virus from Outbreak Cases in Aba'ala District of Afar Region, Ethiopia.Veterinary medicine international · 2020Article
- Model of persistent foot-and-mouth disease virus infection in multilayered cells derived from bovine dorsal soft palate.Transboundary and emerging diseases · 2020Article
- Single-Cell Analysis of the Impact of Host Cell Heterogeneity on Infection with Foot-and-Mouth Disease Virus.Journal of virology · 2018Article
- Isolation and identification of bovine nasopharyngeal mucosal epithelial cells and establishment of cell models of acute infection by foot-and-mouth disease virus.In vitro cellular & developmental biology. Animal · 2018Article
- Cellular response to persistent foot-and-mouth disease virus infection is linked to specific types of alterations in the host cell transcriptome.Scientific reports · 2018Article
- Effect of different culture systems on the production of foot and mouth disease trivalent vaccine.Veterinary world · 2016Article
- Comparative study on the immunopotentiator effect of ISA 201, ISA 61, ISA 50, ISA 206 used in trivalent foot and mouth disease vaccine.Veterinary world · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFoot-and-mouth disease virus (FMDV) is able to cause persistent infection in ruminants besides acute infection and disease. Since the mechanisms of viral persistence and the determining factors are still unknown, in vitro systems help explore and reveal mechanisms of persistence in vivo by providing useful models for the study of RNA genome mutations and evolution. Ammonium chloride, a lysosomotropic agent that raises intralysosomal pH, reduces the yield of FMDV during infection of BHK-21 cells.
resultsThe persistent infection with FMDV serotype O in BHK-21 cells was selected and established readily after treatment of ammonium chloride that acts primarily on the cells. Intact virions were observed located inside the endosomes. Viral genome RNAs and specific proteins were detected in the persistent cells to validate the establishment of viral persistence. Infection of the persistent viruses could not form plaques in host cells but virulence was enhanced. Basing on analysis and comparison of cDNA sequences of resident viruses and wild type viruses, 15 amine acid mutations were found, all of which were located in nonstructural proteins rather than in structural proteins.
conclusionsTherefore, persistent infection of cell cultures with FMDV is successfully established with some distinctive features. It would be worthwhile to further investigate the mechanisms of viral persistence.
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