ArticleJournal of computational neuroscience2011
Sequestration of CaMKII in dendritic spines in silico.
Article in Journal of computational neuroscience, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- Real-time single-molecule imaging of CaMKII-calmodulin interactions.Biophysical journal · 2024Article
- Synaptic memory and CaMKII.Physiological reviews · 2023Review
- Conformational spread drives the evolution of the calcium-calmodulin protein kinase II.Scientific reports · 2022Article
- Incorporating domain growth into hybrid methods for reaction-diffusion systems.Journal of the Royal Society, Interface · 2021Article
- The blending region hybrid framework for the simulation of stochastic reaction-diffusion processes.Journal of the Royal Society, Interface · 2020Article
- Architectural Dynamics of CaMKII-Actin Networks.Biophysical journal · 2019Article
- Active dendrites regulate the spatiotemporal spread of signaling microdomains.PLoS computational biology · 2018Article
- The auxiliary region method: a hybrid method for coupling PDE- and Brownian-based dynamics for reaction-diffusion systems.Royal Society open science · 2018Article
- Spatially extended hybrid methods: a review.Journal of the Royal Society, Interface · 2018Review
- β-adrenergic signaling broadly contributes to LTP induction.PLoS computational biology · 2017Article
- Multiple CaMKII Binding Modes to the Actin Cytoskeleton Revealed by Single-Molecule Imaging.Biophysical journal · 2016Article
- Multiple spatial and kinetic subpopulations of CaMKII in spines and dendrites as resolved by single-molecule tracking PALM.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Article
- Approaches and tools for modeling signaling pathways and calcium dynamics in neurons.Journal of neuroscience methods · 2013Review
- Superresolving dendritic spines.Biophysical journal · 2013Article
- Signaling pathways involved in striatal synaptic plasticity are sensitive to temporal pattern and exhibit spatial specificity.PLoS computational biology · 2013Article
- Spatiotemporal maps of CaMKII in dendritic spines.Journal of computational neuroscience · 2012Article
- Quantitative estimates of the cytoplasmic, PSD, and NMDAR-bound pools of CaMKII in dendritic spines.Brain research · 2011Article
- Molecular mechanisms underlying neuronal synaptic plasticity: systems biology meets computational neuroscience in the wilds of synaptic plasticity.Wiley interdisciplinary reviews. Systems biology and medicineReview
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Calcium calmodulin dependent kinase II (CaMKII) is sequestered in dendritic spines within seconds upon synaptic stimulation. The program Smoldyn was used to develop scenarios of single molecule CaMKII diffusion and binding in virtual dendritic spines. We first validated simulation of diffusion as a function of spine morphology. Additional cellular structures were then incorporated to simulate binding of CaMKII to the post-synaptic density (PSD); binding to cytoskeleton; or their self-aggregation. The distributions of GFP tagged native and mutant constructs in dissociated hippocampal neurons were measured to guide quantitative analysis. Intra-spine viscosity was estimated from fluorescence recovery after photo-bleach (FRAP) of red fluorescent protein. Intra-spine mobility of the GFP-CaMKIIα constructs was measured, with hundred-millisecond or better time resolution, from FRAP of distal spine tips in conjunction with fluorescence loss (FLIP) from proximal regions. Different FRAP \ FLIP profiles were predicted from our Scenarios and provided a means to differentiate binding to the PSDs from self-aggregation. The predictions were validated by experiments. Simulated fits of the Scenarios provided estimates of binding and rate constants. We utilized these values to assess the role of self-aggregation during the initial response of native CaMKII holoenzymes to stimulation. The computations revealed that self-aggregation could provide a concentration-dependent switch to amplify CaMKII sequestration and regulate its activity depending on its occupancy of the actin cytoskeleton.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.