ArticleNucleic acids research2011
Mechanistic analysis of Xenopus EXO1's function in 5'-strand resection at DNA double-strand breaks.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 26 citations in OpenAlex.
- Structure-function analysis of TOPBP1's role in ATR signaling using the DSB-mediated ATR activation in Xenopus egg extracts (DMAX) system.Scientific reports · 2021Article
- CtIP promotes the motor activity of DNA2 to accelerate long-range DNA end resection.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Multiple roles of DNA2 nuclease/helicase in DNA metabolism, genome stability and human diseases.Nucleic acids research · 2020Review
- Sensing and Processing of DNA Interstrand Crosslinks by the Mismatch Repair Pathway.Cell reports · 2017Article
- DNA-damage-induced degradation of EXO1 exonuclease limits DNA end resection to ensure accurate DNA repair.The Journal of biological chemistry · 2017Article
- Ensemble and Single-Molecule Analysis of Non-Homologous End Joining in Frog Egg Extracts.Methods in enzymology · 2017Article
- CDK1 phosphorylates WRN at collapsed replication forks.Nature communications · 2016Article
- The structure of ends determines the pathway choice and Mre11 nuclease dependency of DNA double-strand break repair.Nucleic acids research · 2016Article
- Sharpening the ends for repair: mechanisms and regulation of DNA resection.Acta biochimica et biophysica Sinica · 2016Review
- A Selective Small Molecule DNA2 Inhibitor for Sensitization of Human Cancer Cells to Chemotherapy.EBioMedicine · 2016Article
- MRN, CtIP, and BRCA1 mediate repair of topoisomerase II-DNA adducts.The Journal of cell biology · 2016Article
- DNA double-strand breaks with 5' adducts are efficiently channeled to the DNA2-mediated resection pathway.Nucleic acids research · 2016Article
- The N-terminus of RPA large subunit and its spatial position are important for the 5'->3' resection of DNA double-strand breaks.Nucleic acids research · 2015Article
- 14-3-3 proteins restrain the Exo1 nuclease to prevent overresection.The Journal of biological chemistry · 2015Article
- DNA repair mechanisms and their biological roles in the malaria parasite Plasmodium falciparum.Microbiology and molecular biology reviews : MMBR · 2014Review
- Exonuclease 1 is a critical mediator of survival during DNA double strand break repair in nonquiescent hematopoietic stem and progenitor cells.Stem cells (Dayton, Ohio) · 2014Article
- Human RecQ helicases in DNA repair, recombination, and replication.Annual review of biochemistry · 2014Review
- PCNA promotes processive DNA end resection by Exo1.Nucleic acids research · 2013Article
- Activation of DSB processing requires phosphorylation of CtIP by ATR.Molecular cell · 2013Article
- Analysis of MRE11's function in the 5'-->3' processing of DNA double-strand breaks.Nucleic acids research · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The processing of DNA double-strand breaks (DSBs) into 3' single-stranded tails is the first step of homology-dependent DSB repair. A key player in this process is the highly conserved eukaryotic exonuclease 1 (EXO1), yet its precise mechanism of action has not been rigorously determined. To address this issue, we reconstituted 5'-strand resection in cytosol derived from unfertilized interphase eggs of the frog Xenopus laevis. Xenopus EXO1 (xEXO1) was found to display strong 5'→3' dsDNA exonuclease activity but no significant ssDNA exonuclease activity. Depletion of xEXO1 caused significant inhibition of 5' strand resection. Co-depletion of xEXO1 and Xenopus DNA2 (xDNA2) showed that these two nucleases act in parallel pathways and by distinct mechanisms. While xDNA2 acts on ssDNA unwound mainly by the Xenopus Werner syndrome protein (xWRN), xEXO1 acts directly on dsDNA. Furthermore, xEXO1 and xWRN are required for both the initiation stage and the extension stage of resection. These results reveal important novel information on the mechanism of 5'-strand resection in eukaryotes.
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Registered trials
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