ArticleThe Journal of biological chemistry2011
Introduction of N-linked glycans in the lectin domain of surfactant protein D: impact on interactions with influenza A viruses.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Surfactant protein D: prognostic insights and emerging therapeutic roles in cancer.Frontiers in immunology · 2026Review
- Labeled TEMPO-Oxidized Mannan Differentiates Binding Profiles within the Collectin Families.International journal of molecular sciences · 2022Article
- Changes in the Hemagglutinin and Internal Gene Segments Were Needed for Human Seasonal H3 Influenza A Virus to Efficiently Infect and Replicate in Swine.Pathogens (Basel, Switzerland) · 2022Article
- Viral Evasion of Innate Immune Defense: The Case of Resistance of Pandemic H1N1 Influenza A Virus to Human Mannose-Binding Proteins.Frontiers in microbiology · 2021Article
- Enhanced Antiviral Activity of Human Surfactant Protein D by Site-Specific Engineering of the Carbohydrate Recognition Domain.Frontiers in immunology · 2019Article
- Involvement of Surfactant Protein D in Ebola Virus Infection Enhancement via Glycoprotein Interaction.Viruses · 2018Article
- Lectin-mediated binding and sialoglycans of porcine surfactant protein D synergistically neutralize influenza A virus.The Journal of biological chemistry · 2018Article
- The Role and Molecular Mechanism of Action of Surfactant Protein D in Innate Host Defense Against Influenza A Virus.Frontiers in immunology · 2018Review
- Surfactant Protein D in Respiratory and Non-Respiratory Diseases.Frontiers in medicine · 2018Review
- A Role for Neutrophils in Viral Respiratory Disease.Frontiers in immunology · 2017Review
- Emerging antiviral strategies to interfere with influenza virus entry.Medicinal research reviews · 2014Review
- Comparative evaluation of heterologous production systems for recombinant pulmonary surfactant protein D.Frontiers in immunology · 2014Review
- A unique sugar-binding site mediates the distinct anti-influenza activity of pig surfactant protein D.The Journal of biological chemistry · 2012Article
- Soluble host defense lectins in innate immunity to influenza virus.Journal of biomedicine & biotechnology · 2012Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Porcine surfactant protein D (pSP-D) displays distinctively strong, broad-range inhibitory activity against influenza A virus (IAV). N-Linked glycosylation of the carbohydrate recognition domain (CRD) of pSP-D contributes to the high affinity of this collectin for IAV. To investigate the role of the N-linked glycan further, HEK293E protein expression was used to produce recombinant pSP-D (RpSP-D) that has similar structural and antiviral properties as NpSP-D. We introduced an additional N-linked glycan in the CRD of RpSP-D but this modification did not alter the antiviral activity. Human SP-D is unglycosylated in its CRD and less active against IAV compared with pSP-D. In an attempt to modify its antiviral properties, several recombinant human SP-D (RhSP-D) mutants were constructed with N-linked glycans introduced at various locations within its CRD. To retain lectin activity, necessary for the primary interactions between SP-D and IAV, N-linked glycosylation of RhSP-D was shown to be restricted to the corresponding position in the CRD of either pSP-D or surfactant protein A (SP-A). These N-glycosylated RhSP-D mutants, however, did not show increased neutralization activity against IAV. By developing RhSP-D mutants that also have the pSP-D-specific Ser-Gly-Ala loop inserted in the CRD, we could demonstrate that the N-linked glycan-mediated interactions between pSP-D and IAV involves additional structural prerequisites of the pSP-D CRD. Ultimately, these studies will help to develop highly effective SP-D-based therapeutic and prophylactic drugs against IAV.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.