ArticleVirology journal2011
Functional investigation of grass carp reovirus nonstructural protein NS80.
Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Liquid-liquid phase separation is essential for reovirus viroplasm formation and immune evasion.Journal of virology · 2024Article
- Grass carp reovirus VP56 and VP35 induce formation of viral inclusion bodies for replication.iScience · 2024Article
- Grass Carp Reovirus Induces Formation of Lipid Droplets as Sites for Its Replication and Assembly.mBio · 2022Article
- Identification, Virulence, and Molecular Characterization of a Recombinant Isolate of Grass Carp Reovirus Genotype I.Viruses · 2021Article
- Structure and function of S9 segment of grass carp reovirus Anhui strain.Virusdisease · 2017Article
- Identification and characterization of two cleavage fragments from the Aquareovirus nonstructural protein NS80.Virologica Sinica · 2016Article
- Screening and analysis on the protein interaction of the protein VP7 in grass carp reovirus.Virus genes · 2015Article
- Pathogenicity and tissue distribution of grass carp reovirus after intraperitoneal administration.Virology journal · 2014Article
- Molecular cloning of grass carp (Ctenopharyngodon idellus) T-bet and GATA-3, and their expression profiles with IFN-γ in response to grass carp reovirus (GCRV) infection.Fish physiology and biochemistry · 2013Article
- Aquareovirus protein VP6 colocalizes with NS80 protein in infected and transfected cells.Virology journal · 2013Article
- Nonstructural protein NS80 is crucial in recruiting viral components to form aquareoviral factories.PloS one · 2013Article
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- Characterization of grass carp reovirus minor core protein VP4.Virology journal · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGrass Carp Reovirus (GCRV), a highly virulent agent of aquatic animals, has an eleven segmented dsRNA genome encased in a multilayered capsid shell, which encodes twelve proteins including seven structural proteins (VP1-VP7), and five nonstructural proteins (NS80, NS38, NS31, NS26, and NS16). It has been suggested that the protein NS80 plays an important role in the viral replication cycle that is similar to that of its homologous protein μNS in the genus of Orthoreovirus.
resultsAs a step to understanding the basis of the part played by NS80 in GCRV replication and particle assembly, we used the yeast two-hybrid (Y2H) system to identify NS80 interactions with proteins NS38, VP4, and VP6 as well as NS80 and NS38 self-interactions, while no interactions appeared in the four protein pairs NS38-VP4, NS38-VP6, VP4-VP4, and VP4-VP6. Bioinformatic analyses of NS80 with its corresponding proteins were performed with all currently available homologous protein sequences in ARVs (avian reoviruses) and MRVs (mammalian reoviruses) to predict further potential functional domains of NS80 that are related to VFLS (viral factory-like structures) formation and other roles in viral replication. Two conserved regions spanning from aa (amino acid) residues of 388 to 433, and 562 to 580 were discovered in this study. The second conserved region with corresponding conserved residues Tyr565, His569, Cys571, Asn573, and Glu576 located between the two coiled-coils regions (aa ~513-550 and aa ~615-690) in carboxyl-proximal terminus were supposed to be essential to form VFLS, so that aa residues ranging from 513 to 742 of NS80 was inferred to be the smallest region that is necessary for forming VFLS. The function of the first conserved region including Ala395, Gly419, Asp421, Pro422, Leu438, and Leu443 residues is unclear, but one-third of the amino-terminal region might be species specific, dominating interactions with other viral components.
conclusionsOur results in this study together with those from previous investigations indicate the protein NS80 might play a central role in VFLS formation and viral components recruitment in GCRV particle assembly, similar to the μNS protein in ARVs and MRVs.
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