ArticleRadiation oncology (London, England)2011
Survival analysis of F98 glioma rat cells following minibeam or broad-beam synchrotron radiation therapy.
Article in Radiation oncology (London, England), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 26 citations in OpenAlex.
- Time-lapse imaging of cells in spatially fractionated X-ray fields using a mini beam as an alternative to accelerator-based sub-millimeter beams.Journal of radiation research · 2025Article
- Superior Anti-Tumor Response After Microbeam and Minibeam Radiation Therapy in a Lung Cancer Mouse Model.Cancers · 2025Article
- Comprehensive dosimetric commissioning of proton minibeam radiotherapy on a single gantry proton system.Frontiers in oncology · 2024Article
- Oleic Acid Protects Endothelial Cells from Silica-Coated Superparamagnetic Iron Oxide Nanoparticles (SPIONs)-Induced Oxidative Stress and Cell Death.International journal of molecular sciences · 2022Article
- A proof of principle experiment for microbeam radiation therapy at the Munich compact light source.Radiation and environmental biophysics · 2020Article
- Small GTP-binding protein PdRanBP regulates vascular tissue development in poplar.BMC genetics · 2016Article
- Nanotube x-ray for cancer therapy: a compact microbeam radiation therapy system for brain tumor treatment.Expert review of anticancer therapy · 2014Review
- Physiologically gated microbeam radiation using a field emission x-ray source array.Medical physics · 2014Article
- Double-strand breaks on F98 glioma rat cells induced by minibeam and broad-beam synchrotron radiation therapy.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2014Article
- Image-guided microbeam irradiation to brain tumour bearing mice using a carbon nanotube x-ray source array.Physics in medicine and biology · 2014Article
- Resazurin-based assay for screening bacteria for radiation sensitivity.SpringerPlus · 2013Article
- Synchrotron radiation in cancer treatments and diagnostics: an overview.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2011Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn the quest of a curative radiotherapy treatment for gliomas new delivery modes are being explored. At the Biomedical Beamline of the European Synchrotron Radiation Facility (ESRF), a new spatially-fractionated technique, called Minibeam Radiation Therapy (MBRT) is under development. The aim of this work is to compare the effectiveness of MBRT and broad-beam (BB) synchrotron radiation to treat F98 glioma rat cells. A dose escalation study was performed in order to delimit the range of doses where a therapeutic effect could be expected. These results will help in the design and optimization of the forthcoming in vivo studies at the ESRF.
methodsTwo hundred thousand F98 cells were seeded per well in 24-well plates, and incubated for 48 hours before being irradiated with spatially fractionated and seamless synchrotron x-rays at several doses. The percentage of each cell population (alive, early apoptotic and dead cells, where either late apoptotic as necrotic cells are included) was assessed by flow cytometry 48 hours after irradiation, whereas the metabolic activity of surviving cells was analyzed on days 3, 4, and 9 post-irradiation by using QBlue test.
resultsThe endpoint (or threshold dose from which an important enhancement in the effectiveness of both radiation treatments is achieved) obtained by flow cytometry could be established just before 12 Gy in the two irradiation schemes, whilst the endpoints assessed by the QBlue reagent, taking into account the cell recovery, were set around 18 Gy in both cases. In addition, flow cytometric analysis pointed at a larger effectiveness for minibeams, due to the higher proportion of early apoptotic cells.
conclusionsWhen the valley doses in MBRT equal the dose deposited in the BB scheme, similar cell survival ratio and cell recovery were observed. However, a significant increase in the number of early apoptotic cells were found 48 hours after the minibeam radiation in comparison with the seamless mode.
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