ArticleMolecular microbiology2011
Circuitry linking the Csr and stringent response global regulatory systems.
Article in Molecular microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 111 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
111 citing papers in PubMed.
- Integrated Transcriptomic and Proteomic Analyses Reveal CsrA-Mediated Regulation of Virulence and Metabolism inMicroorganisms · 2025Article
- Identification of an ArgR-controlled promoter within the outermost region of the ISJournal of bacteriology · 2024Article
- In Silico Analysis of the Phylogenetic and Physiological Characteristics of Sphingobium indicum B90A: A Hexachlorocyclohexane-Degrading Bacterium.Current microbiology · 2024Article
- Multitier regulation of theJournal of bacteriology · 2024Article
- The Post-Transcriptional Regulatory Protein CsrA Amplifies Its Targetome through Direct Interactions with Stress-Response Regulatory Hubs: The EvgA and AcnA Cases.Microorganisms · 2024Article
- Identification and Regulatory Roles of a New Csr Small RNA from Arctic Pseudoalteromonas fuliginea BSW20308 in Temperature Responses.Microbiology spectrum · 2023Article
- Article
- Dual role of CsrA in regulating the hemolytic activity ofVirulence · 2022Article
- Spatial Structure Formation by RsmE-Regulated Extracellular Secretions in Pseudomonas fluorescens Pf0-1.Journal of bacteriology · 2022Article
- Key players in regulatory RNA realm of bacteria.Biochemistry and biophysics reports · 2022Review
- Review
- DnaA and SspA regulation of the iraD gene of Escherichia coli: an alternative DNA damage response independent of LexA/RecA.Genetics · 2022Article
- CsrA regulation via binding to the base-pairing small RNA Spot 42.Molecular microbiology · 2022Article
- Article
- Amplifying and Fine-Tuning Rsm sRNAs Expression and Stability to Optimize the Survival ofMicroorganisms · 2021Article
- Single-Target Regulators Constitute the Minority Group of Transcription Factors inFrontiers in microbiology · 2021Article
- Genome-Wide Analysis of Targets for Post-Transcriptional Regulation by Rsm Proteins inFrontiers in molecular biosciences · 2021Article
- Protein dosage of theJournal of bacteriology · 2020Article
- CsrA Supports both Environmental Persistence and Host-Associated Growth of Acinetobacter baumannii.Infection and immunity · 2020Article
- Rewiring of growth-dependent transcription regulation by a point mutation in region 1.1 of the housekeeping σ factor.Nucleic acids research · 2020Article
51 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
Abstract
CsrA protein regulates important cellular processes by binding to target mRNAs and altering their translation and/or stability. In Escherichia coli, CsrA binds to sRNAs, CsrB and CsrC, which sequester CsrA and antagonize its activity. Here, mRNAs for relA, spoT and dksA of the stringent response system were found among 721 different transcripts that copurified with CsrA. Many of the transcripts that copurified with CsrA were previously determined to respond to ppGpp and/or DksA. We examined multiple regulatory interactions between the Csr and stringent response systems. Most importantly, DksA and ppGpp robustly activated csrB/C transcription (10-fold), while they modestly activated csrA expression. We propose that CsrA-mediated regulation is relieved during the stringent response. Gel shift assays confirmed high affinity binding of CsrA to relA mRNA leader and weaker interactions with dksA and spoT. Reporter fusions, qRT-PCR and immunoblotting showed that CsrA repressed relA expression, and (p)ppGpp accumulation during stringent response was enhanced in a csrA mutant. CsrA had modest to negligible effects on dksA and spoT expression. Transcription of dksA was negatively autoregulated via a feedback loop that tended to mask CsrA effects. We propose that the Csr system fine-tunes the stringent response and discuss biological implications of the composite circuitry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.