ArticleIn vitro cellular & developmental biology. Animal2011
Effects of humic acids in vitro.
Article in In vitro cellular & developmental biology. Animal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 65 citations in OpenAlex.
- Protective Effects of Humic Acid on Intestinal Barrier Dysfunction and Inflammatory Activation in Canine Cell-Based Models.Animals : an open access journal from MDPI · 2026Article
- Pharmacological Effects of Humic Substances and Their Signaling Mechanisms.Molecules (Basel, Switzerland) · 2025Review
- Biomedical Applications of Humic Substances: From Natural Biopolymers to Therapeutic Agents.Antioxidants (Basel, Switzerland) · 2025Review
- Humic acid from vermicompost effectively regulates the redox status and mitigates the progression of experimental periodontitis.Naunyn-Schmiedeberg's archives of pharmacology · 2025Article
- Assessing the safety of thermal mineral water for cosmetic applications: an integrated approach using physicochemical, cheminformatics, and bioinformatics techniques.RSC advances · 2025Article
- Humic Acid Derived from Vermicompost Improves Bone Mineral Content and Alters Oxidative Stress Markers in Ovariectomized Mice.Biomedicines · 2025Article
- Humic Acid Derived from Agricultural Biomass Mitigates Alveolar Bone Loss and Modulates Systemic Inflammatory Cytokines in Rats with Periodontitis.Current topics in medicinal chemistry · 2025Article
- Effect of Modified Forms of Sodium Humate PowHumus on the Balance of Arginine in Peritoneal Macrophages of Intact Mice.Bulletin of experimental biology and medicine · 2024Article
- The Role of Intracellular Signaling Molecules in Macrophage Activation by Peat Humic Acids.Bulletin of experimental biology and medicine · 2023Article
- Therapeutic Efficiency of Humic Acids in Intoxications.Life (Basel, Switzerland) · 2023Review
- Humic Substances as a Feed Supplement and the Benefits of Produced Chicken Meat.Life (Basel, Switzerland) · 2023Review
- Natural Compounds and Products from an Anti-Aging Perspective.Molecules (Basel, Switzerland) · 2022Review
- Quantitative Structure-Activity Relationship, Ontology-Based Model of the Antioxidant and Cell Protective Activity of Peat Humic Acids.Polymers · 2022Article
- Influence of Sodium Humate on the Growth Performance, Diarrhea Incidence, Blood Parameters, and Fecal Microflora of Pre-Weaned Dairy Calves.Animals : an open access journal from MDPI · 2022Article
- A Systematic Study of the Antioxidant Capacity of Humic Substances against Peroxyl Radicals: Relation to Structure.Polymers · 2021Article
- The effect of humic acid in chronic deoxynivalenol intoxication.Environmental science and pollution research international · 2021Article
- Effects of Dietary Supplementation of Humic Acid Sodium and Zinc Oxide on Growth Performance, Immune Status and Antioxidant Capacity of Weaned Piglets.Animals : an open access journal from MDPI · 2020Article
- Effect of humic acids on lead poisoning in bones and on a subcellular level in mitochondria.Environmental science and pollution research international · 2020Article
- A toxicological evaluation of a fulvic and humic acids preparation.Toxicology reports · 2020Article
- Humic acid inhibits HBV-induced autophagosome formation and induces apoptosis in HBV-transfected Hep G2 cells.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Humic acids are known for their overall positive health and productivity effects in animal feeding trials and, controversially, as an aetiological factor of cancer. We tried to assess the in vitro effect of humic acids from a selected source in Slovakia when used at recommended prophylactic dosage. We investigated antioxidant properties, enzymatic and non-enzymatic antioxidant defence system in liver mitochondria and cultured cancer cell lines in vitro. We observed a significant decrease in superoxide dismutase activity after humic acids treatment irrespective of dissolving in dimethyl sulphoxide or direct addition to mitochondria suspension in a respiration medium. Activities of other antioxidant enzymes measured, such as glutathione peroxidase and glutathione reductase, showed no significant differences from the control as well as the reduced glutathione content. Percentage of inhibition by humic acids of superoxide radical indicated lower efficacy compared with that of hydroxyl radical. Survival of six different cancer cells lines indicated that only the acute T lymphoblastic leukaemia cell line was sensitive to the tested humic acids. Despite relatively low solubility in aqueous solutions, humic acids from the selected source participated in redox regulation. By recapturing the radicals, humic acids reloaded the antioxidant defensive mechanism. Results from in vitro study conducted with humic acids from the natural source showed potential of these substances as promising immunity enhancing agents.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.