ArticleThe Journal of biological chemistry2011
Hypoxia induces an increase in intracellular magnesium via transient receptor potential melastatin 7 (TRPM7) channels in rat hippocampal neurons in vitro.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- Magnesium (MgCurrent pharmaceutical design · 2024Review
- CaInternational journal of molecular sciences · 2022Review
- Suppression of TRPM7 Inhibited Hypoxia-Induced Migration and Invasion of Androgen-Independent Prostate Cancer Cells by Enhancing RACK1-Mediated Degradation of HIF-1Oxidative medicine and cellular longevity · 2020Article
- Role of the chanzyme TRPM7 in the nervous system in health and disease.Cellular and molecular life sciences : CMLS · 2019Review
- MicroRNA‑217 inhibition relieves cerebral ischemia/reperfusion injury by targeting SIRT1.Molecular medicine reports · 2019Article
- Magnesium Is a Key Player in Neuronal Maturation and Neuropathology.International journal of molecular sciences · 2019Review
- Deregulated renal magnesium transport during lipopolysaccharide-induced acute kidney injury in mice.Pflugers Archiv : European journal of physiology · 2019Article
- Effects of TRPM7/miR-34a Gene Silencing on Spatial Cognitive Function and Hippocampal Neurogenesis in Mice with Type 1 Diabetes Mellitus.Molecular neurobiology · 2018Article
- A researcher's guide to the galaxy of IRESs.Cellular and molecular life sciences : CMLS · 2017Review
- Nuclear translocation of annexin 1 following oxygen-glucose deprivation-reperfusion induces apoptosis by regulating Bid expression via p53 binding.Cell death & disease · 2016Article
- Article
- Neuronal acid-induced [Zn²⁺]i elevations calibrated using the low-affinity ratiometric probe FuraZin-1.Journal of neurochemistry · 2015Article
- Following OGD/R, annexin 1 nuclear translocation and subsequent induction of apoptosis in neurons are assisted by myosin IIA in a TRPM7 kinase-dependent manner.Molecular neurobiology · 2015Article
- Molecular determinants of magnesium-dependent synaptic plasticity at electrical synapses formed by connexin36.Nature communications · 2014Article
- Proton-sensitive cation channels and ion exchangers in ischemic brain injury: new therapeutic targets for stroke?Progress in neurobiology · 2014Review
- TRPM7.Handbook of experimental pharmacology · 2014Review
- TRPM7, the cytoskeleton and neuronal death.Channels (Austin, Tex.) · 2013Review
- A key role for Mg(2+) in TRPM7's control of ROS levels during cell stress.The Biochemical journal · 2012Article
- TRPM channels: same ballpark, different players, and different rules in immunogenetics.Immunogenetics · 2011Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
TRPM7, a divalent cation channel, plays an important role in neurons damaged from cerebral ischemia due to permitting intracellular calcium overload. This study aimed to explore whether magnesium was transported via a TRPM7 channel into the intracellular space of rat hippocampal neurons after 1 h of oxygen-glucose deprivation (OGD) and acute chemical ischemia (CI) by using methods of the Mg(2+) fluorescent probe Mag-Fura-2 to detect intracellular magnesium concentration ([Mg(2+)](i)) and flame atomic absorption spectrometry to measure extracellular magnesium concentration ([Mg(2+)](o)). The results showed that the neuronal [Mg(2+)](i) was 1.51-fold higher after 1 h of OGD at a basal level, and the increase of neuronal [Mg(2+)](i) reached a peak after 1 h of OGD and was kept for 60 min with re-oxygenation. Meanwhile, the [Mg(2+)](o) decreased after 1 h of OGD and recovered to the pre-ischemic level within 15 min after re-oxygenation. In the case of CI, the [Mg(2+)](i) peak immediately appeared in hippocampal neurons. This increase of [Mg(2+)](i) declined by removing extracellular magnesium in OGD or CI. Furthermore, by using Gd(3+) or 2-aminoethoxydiphenyl borate to inhibit TRPM7 channels, the [Mg(2+)](i) increase, which was induced by OGD or CI, was attenuated without altering the basal level of [Mg(2+)](i). By silencing TRPM7 with shRNA in hippocampal neurons, it was found that not only was the increase of [Mg(2+)](i) induced by OGD or CI but also the basal levels of [Mg(2+)](i) were attenuated. In contrast, overexpression of TRPM7 in HEK293 cells exaggerated both the basal levels and increased [Mg(2+)](i) after 1 h of OGD/CI. These results suggest that anoxia induced the increase of [Mg(2+)](i) via TRPM7 channels in rat hippocampal neurons.
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