ArticleThe Journal of physiology2011
In vivo two-photon uncaging of glutamate revealing the structure-function relationships of dendritic spines in the neocortex of adult mice.
Article in The Journal of physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.
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Who cites it
110 citing papers in PubMed, 179 citations in OpenAlex.
- Ethological learning during the critical period resets synaptic setpoints in mouse binocular visual cortex.Neuron · 2026Article
- Functional organization of dendritic spines in mouse visual cortex layer 2/3 neurons.iScience · 2026Article
- Overexpression of Synaptopodin increases the number of spine apparatuses and active synapses of dentate granule cells.Scientific reports · 2026Article
- Functional synaptic connectivity shapes spine stability in the hippocampus.Nature communications · 2026Article
- Loss of Necdin causes social deficit and aberrant synaptic function through destabilization of SynGAP.Molecular psychiatry · 2026Article
- Widely Used CaMKII Regulatory Segment Mutations Cause Tight Actinin Binding and Dendritic Spine Enlargement in Unstimulated Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Molecular Tools to Study and Control Dopaminergic Neurotransmission With Light.Medicinal research reviews · 2025Review
- Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution.Communications biology · 2025Article
- The Histone Lysine Demethylase KDM7A Contributes to Reward Memory via Fscn1-Induced Synaptic Plasticity in the Medial Prefrontal Cortex.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
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- Focal clusters of peri-synaptic matrix contribute to activity-dependent plasticity and memory in mice.Cell reports · 2024Article
- Fluorescence microscopy shadow imaging for neuroscience.Frontiers in cellular neuroscience · 2024Review
- Current Status and Future Strategies for Advancing Functional Circuit MappingThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Rac1 GTPase activation impairs fear conditioning-induced structural changes in basolateral amygdala neurons and long-term fear memory formation.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2023Article
- In vivo photopharmacology with a caged mu opioid receptor agonist drives rapid changes in behavior.Nature methods · 2023Article
- Advances in the Electrophysiological Recordings of Long-Term Potentiation.International journal of molecular sciences · 2023Review
- Optical control of neuronal activities with photoswitchable nanovesicles.Nano research · 2023Article
- Dendritic Spines in Learning and Memory: From First Discoveries to Current Insights.Advances in neurobiology · 2023Article
- Bayesian reconstruction of memories stored in neural networks from their connectivity.PLoS computational biology · 2023Article
- Electrophysiology of Dendritic Spines: Information Processing, Dynamic Compartmentalization, and Synaptic Plasticity.Advances in neurobiology · 2023Article
50 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 2 institutions in 2 countries.
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Abstract
Two-photon (2P) uncaging of caged neurotransmitters can efficiently stimulate individual synapses and is widely used to characterize synaptic functions in brain slice preparations. Here we extended 2P uncaging to neocortical pyramidal neurons in adult mice in vivo where caged glutamate was applied from the pial surface. To validate the methodology, we applied a small fluorescent probe using the same method, and confirmed that its concentrations were approximately homogenous up to 200 μm below the cortical surface, and that the extracellular space of the neocortex was as large as 22%. In fact, in vivo whole-cell recording revealed that 2P glutamate uncaging could elicit transient currents (2pEPSCs) very similar to excitatory postsynaptic currents (EPSCs). A spatial resolution of glutamate uncaging was 0.6-0.8 μm up to the depth of 200 μm, and in vivo 2P uncaging was able to stimulate single identified spines. Automated three-dimensional (3-D) mapping of such 2pEPSCs which covered the surfaces of dendritic branches revealed that functional AMPA receptor expression was stable and proportional to spine volume.Moreover, in vivo 2P Ca2+ imaging and uncaging suggested that the amplitudes of glutamate-induced Ca2+ transients were inversely proportional to spine volume. Thus, the key structure-function relationships hold in dendritic spines in adult neocortex in vivo, as in young hippocampal slice preparations. In vivo 2P uncaging will be a powerful tool to investigate properties of synapses in the neocortex.
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