ArticleBMC microbiology2011
Community analysis of bacteria colonizing intestinal tissue of neonates with necrotizing enterocolitis.
Article in BMC microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 1 of them a synthesis that pooled it.
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Who cites it
47 citing papers in PubMed, 1 synthesis or guideline pooled it, 99 citations in OpenAlex.
- Pooled it
- Necrotizing enterocolitis.Nature reviews. Disease primers · 2026Review
- Transcriptome, metabolome, and inflammatory and oxidative properties ofApplied and environmental microbiology · 2026Article
- Ralstonia pickettii as an emerging pediatric pathogen: a mini-review of current evidence.Frontiers in pediatrics · 2026Review
- Boric Acid in Milk Replacer as a Health Enhancer and Growth Promoter for Lambs in the Suckling Period.Biological trace element research · 2025Article
- Molecular testing for gastrointestinal pathogens in intestinal tissue of infants with necrotizing enterocolitis or spontaneous intestinal perforation.Journal of perinatology : official journal of the California Perinatal Association · 2024Article
- Role of the microbiome in pathophysiology of necrotising enterocolitis in preterm neonates.BMJ paediatrics open · 2023Review
- Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling.Nature protocols · 2023Review
- Bacillus velezensis DSM 33864 reduces Clostridioides difficile colonization without disturbing commensal gut microbiota composition.Scientific reports · 2023Article
- Variability in antibiotic duration for necrotizing enterocolitis and outcomes in a large multicenter cohort.Journal of perinatology : official journal of the California Perinatal Association · 2022Article
- Interpretable prediction of necrotizing enterocolitis from machine learning analysis of premature infant stool microbiota.BMC bioinformatics · 2022Article
- Clinical outcomes and gestational age based prediction of pneumatosis intestinalis in preterm infants with necrotizing enterocolitis.Journal of neonatal-perinatal medicine · 2022Article
- Technical challenges regarding the use of formalin-fixed paraffin embedded (FFPE) tissue specimens for the detection of bacterial alterations in colorectal cancer.BMC microbiology · 2021Article
- Microbiome analysis reveals gut microbiota alteration of early-weaned Yimeng black goats with the effect of milk replacer and age.Microbial cell factories · 2021Article
- Article
- Early-life gut dysbiosis linked to juvenile mortality in ostriches.Microbiome · 2020Article
- Precision-based modeling approaches for necrotizing enterocolitis.Disease models & mechanisms · 2020Review
- Molecular epidemiology ofNew microbes and new infections · 2019Article
- Using formalin fixed paraffin embedded tissue to characterize the preterm gut microbiota in necrotising enterocolitis and spontaneous isolated perforation using marginal and diseased tissue.BMC microbiology · 2019Article
- Metabolomic signatures distinguish the impact of formula carbohydrates on disease outcome in a preterm piglet model of NEC.Microbiome · 2018Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNecrotizing enterocolitis (NEC) is the most common gastrointestinal emergency in newborn neonates. Bacteria are believed to be important in the pathogenesis of NEC but bacterial characterization has only been done on human faecal samples and experimental animal studies. The aim of this study was to investigate the microbial composition and the relative number of bacteria in inflamed intestinal tissue surgically removed from neonates diagnosed with NEC (n=24). The bacterial populations in the specimens were characterized by laser capture microdissection and subsequent sequencing combined with fluorescent in situ hybridization (FISH), using bacterial rRNA-targeting oligonucleotide probes.
resultsBacteria were detected in 22 of the 24 specimens, 71% had moderate to high densities of bacteria. The phyla detected by 16S rRNA gene sequencing were: Proteobacteria (49.0%), Firmicutes (30.4%), Actinobacteria (17.1%) and Bacteroidetes (3.6%). A major detected class of the phylum Proteobacteria belonged to δ-proteobacteria. Surprisingly, Clostridium species were only detected in 4 of the specimens by FISH, but two of these specimens exhibited histological pneumatosis intestinalis and both specimens had a moderate to a high density of C. butyricum and C. parputrificum detected by using species specific FISH probes. A 16S rRNA gene sequence tag similar to Ralstonia species was detected in most of the neonatal tissues and members of this genus have been reported to be opportunistic pathogens but their role in NEC has still to be clarified.
conclusionIn this study, in situ identification and community analysis of bacteria found in tissue specimens from neonates with NEC, were analysed for the first time. Although a large variability of bacteria was found in most of the analyzed specimens, no single or combination of known potential pathogenic bacteria species was dominating the samples suggestive NEC as non-infectious syndrome. However there was a significant correlation between the presence of C. butyricum & C. parputrificum and histological pneumatosis intestinalis. Finally this study emphasizes the possibility to examine the microbial composition directly on excised human tissues to avoid biases from faecal samples or culturing.
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