ArticleThe Journal of cell biology1990
Biochemical and immunochemical analysis of rat brain dynamin interaction with microtubules and organelles in vivo and in vitro.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 112 citations in OpenAlex.
- Dynamins maintain nuclear envelope homeostasis and genome stability.Nature communications · 2026Article
- A deep intronic variant in DNM1 in a patient with developmental and epileptic encephalopathy creates a splice acceptor site and affects only transcript variants including exon 10a.Neurogenetics · 2023Article
- DYN-1/dynamin regulates microtubule dynamics after axon injury.microPublication biology · 2022Article
- The model of local axon homeostasis - explaining the role and regulation of microtubule bundles in axon maintenance and pathology.Neural development · 2019Review
- Impact of dynamin 2 on adenovirus nuclear entry.Virology · 2019Article
- Actin dynamics at focal adhesions: a common endpoint and putative therapeutic target for proteinuric kidney diseases.Kidney international · 2018Review
- Activation of the Small G Protein Arf6 by Dynamin2 through Guanine Nucleotide Exchange Factors in Endocytosis.Scientific reports · 2015Article
- Disruption of microtubule network rescues aberrant actin comets in dynamin2-depleted cells.PloS one · 2011Article
- Dynamic instability of microtubules requires dynamin 2 and is impaired in a Charcot-Marie-Tooth mutant.The Journal of cell biology · 2009Article
- Interaction of Mint2 with TrkA is involved in regulation of nerve growth factor-induced neurite outgrowth.The Journal of biological chemistry · 2009Article
- The large GTPase dynamin regulates actin comet formation and movement in living cells.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- DEF-1, a novel Src SH3 binding protein that promotes adipogenesis in fibroblastic cell lines.Molecular and cellular biology · 1999Article
- Essential role of the dynamin pleckstrin homology domain in receptor-mediated endocytosis.Molecular and cellular biology · 1999Article
- Contribution of the GTPase domain to the subcellular localization of dynamin in the nematode Caenorhabditis elegans.Molecular biology of the cell · 1998Article
- A human dynamin-related protein controls the distribution of mitochondria.The Journal of cell biology · 1998Article
- Differential distribution of dynamin isoforms in mammalian cells.Molecular biology of the cell · 1998Article
- Dynamin-mediated internalization of caveolae.The Journal of cell biology · 1998Article
- A novel dynamin-like protein associates with cytoplasmic vesicles and tubules of the endoplasmic reticulum in mammalian cells.The Journal of cell biology · 1998Article
- Specific role for the PH domain of dynamin-1 in the regulation of rapid endocytosis in adrenal chromaffin cells.The EMBO journal · 1997Article
- The dynamins: redundant or distinct functions for an expanding family of related GTPases?Proceedings of the National Academy of Sciences of the United States of America · 1997Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have purified a 100-kD rat brain protein that has microtubule cross-linking activity in vitro, and have determined that it is dynamin, a putative microtubule-associated motility protein. We find that dynamin appears to be specific to neuronal tissue where it is present in both soluble and particulate tissue fractions. In the cytosol it is abundant, representing as much as 1.5% of the total extractable protein. Dynamin appears to be in particulate material due to association with a distinct subcellular membrane fraction. Surprisingly, by immunofluorescence analysis of PC12 cells we find that dynamin is distributed uniformly throughout the cytoplasm with no apparent microtubule association in either interphase, mitotic, or taxol-treated cells. Upon nerve growth factor (NGF) induction of PC12 cell differentiation into neurons, dynamin levels increase approximately twofold. In the cell body, the distribution of dynamin again remains clearly distinct from that of tubulin, and in axons, where microtubules are numerous and ordered into bundles, dynamin staining is sparse and punctate. On the other hand, in the most distal domain of growth cones, where there are relatively few microtubules, dynamin is particularly abundant. The dynamin staining of neurites is abolished by extraction of the cells with detergent under conditions that preserve microtubules, suggesting that dynamin in neurites is associated with membranes. We conclude that dynamin is a neuronal protein that is specifically associated with as yet unidentified vesicles. It is possible, but unproven, that it may link vesicles to microtubules for transport in differentiated axons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.