ArticlePloS one2011
Actomyosin-dependent cortical dynamics contributes to the prophase force-balance in the early Drosophila embryo.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- Nonmuscle myosin IIA (NMIIA) regulates anisotropic cell tension to maintain the hexagonal packing of mouse lens meridional row cells.Molecular biology of the cell · 2025Article
- Reshaping the Syncytial Drosophila Embryo with Cortical Actin Networks: Four Main Steps of Early Development.Results and problems in cell differentiation · 2024Review
- Cellular dynamics of coenocytic endosperm development in Arabidopsis thaliana.Nature plants · 2023Article
- Terminally differentiated osteoclasts organize centrosomes into large clusters for microtubule nucleation and bone resorption.Molecular biology of the cell · 2022Article
- Going with the flow: insights fromPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Bidirectional motility of kinesin-5 motor proteins: structural determinants, cumulative functions and physiological roles.Cellular and molecular life sciences : CMLS · 2018Review
- Two consecutive microtubule-based epithelial seaming events mediate dorsal closure in the scuttle flyeLife · 2018Article
- A potential physiological role for bi-directional motility and motor clustering of mitotic kinesin-5 Cin8 in yeast mitosis.Journal of cell science · 2017Article
- Fluctuation Analysis of Centrosomes Reveals a Cortical Function of Kinesin-1.Biophysical journal · 2015Article
- The microtubule cross-linker Feo controls the midzone stability, motor composition, and elongation of the anaphase B spindle in Drosophila embryos.Molecular biology of the cell · 2015Article
- Let's huddle to prevent a muddle: centrosome declustering as an attractive anticancer strategy.Cell death and differentiation · 2012Review
- Rotational model for actin filament alignment by myosin.Journal of theoretical biology · 2012Article
- And the dead shall rise: actin and myosin return to the spindle.Developmental cell · 2011Review
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundThe assembly of the Drosophila embryo mitotic spindle during prophase depends upon a balance of outward forces generated by cortical dynein and inward forces generated by kinesin-14 and nuclear elasticity. Myosin II is known to contribute to the dynamics of the cell cortex but how this influences the prophase force-balance is unclear. PRINCIPAL
findingsHere we investigated this question by injecting the myosin II inhibitor, Y27632, into early Drosophila embryos. We observed a significant increase in both the area of the dense cortical actin caps and in the spacing of the spindle poles. Tracking of microtubule plus ends marked by EB1-GFP and of actin at the cortex revealed that astral microtubules can interact with all regions of these expanded caps, presumably via their interaction with cortical dynein. In Scrambled mutants displaying abnormally small actin caps but normal prophase spindle length in late prophase, myosin II inhibition produced very short spindles.
conclusionsThese results suggest that two complementary outward forces are exerted on the prophase spindle by the overlying cortex. Specifically, dynein localized on the mechanically firm actin caps and the actomyosin-driven contraction of the deformable soft patches of the actin cortex, cooperate to pull astral microtubules outward. Thus, myosin II controls the size and dynamic properties of the actin-based cortex to influence the spacing of the poles of the underlying spindle during prophase.
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